Construction and performance evaluation of fully biomimetic hyaline cartilage matrix scaffolds for joint defect regeneration.

Autor:	Sun H; School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, People's Republic of China., Wu Z; School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, People's Republic of China., Liu L; School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, People's Republic of China., Hu X; Department of Biomedical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong Special Administrative Region of China 999077, People's Republic of China., Zhao Y; School of Nursing and School of Public Health, Yangzhou University, Yangzhou 225001, People's Republic of China., Wang C; School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, People's Republic of China., Yang J; Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, People's Republic of China., Gu Z; School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, People's Republic of China., Wang DA; Department of Biomedical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong Special Administrative Region of China 999077, People's Republic of China., Yao H; School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu 225009, People's Republic of China.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Oct 28; Vol. 19 (6). Date of Electronic Publication: 2024 Oct 28.
DOI:	10.1088/1748-605X/ad884f
Abstrakt:	Due to the absence of nerves and blood vessels in articular cartilage, its regeneration and repair present a significant and complex challenge in osteoarthritis treatment. Developing a specialized physical and chemical microenvironment supporting cell growth has been difficult in cartilage grafting, especially when aiming for comprehensive biomimetic solutions. Based on previous research, we have designed a tissue-engineered decellularized living hyaline cartilage graft (dLhCG). The study developed a method to improve the hydrophilicity and stiffness of scaffolds by employing chemical grafting techniques and designed a decellularized hyaline cartilage phenotype matrix scaffold for tissue engineering. Here, we reported a method using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride /N-hydroxysuccinimide (EDC/NHS) to achieve the grafting of chondroitin sulfate (CS) onto dLhCG, ultimately producing a tissue-engineered hyaline cartilage graft with the CS (dLhCG/CS). Young's modulus measurements revealed that the cross-linked scaffolds exhibited enhanced mechanical properties. We implanted the cross-linked dLhCG/CS scaffolds into the trochlear region of rat joints and evaluated their functionality through histological analysis and biomechanical tests. After 12 weeks, the dLhCG/CS scaffolds demonstrated excellent bioinductive activity comparable to dLhCG. The regenerated tissue effectively maintained a hyaline cartilage phenotype and exhibited similar mechanical properties, playing a crucial role in cartilage regeneration. (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)
Databáze:	MEDLINE
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