A ring resonators optical sensor for multiple biomarkers detection.

Autor: Favaretto R; FTH srl, Via Sommarive 18, 38123, Trento, Italy; Department of Physics, University of Trento, Via Sommarive 14, 38123, Trento, Italy., Ardoino N; FTH srl, Via Sommarive 18, 38123, Trento, Italy., Pucker G; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy., Bellotto N; FTH srl, Via Sommarive 18, 38123, Trento, Italy., Mancinelli M; Department of Physics, University of Trento, Via Sommarive 14, 38123, Trento, Italy., Piccoli G; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy., Bernard M; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy., Vanzetti L; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy., Potrich C; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy; CNR Institute of Biophysics, Via alla Cascata 56, Povo, 38123, Trento, Italy., Lunelli L; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy; CNR Institute of Biophysics, Via alla Cascata 56, Povo, 38123, Trento, Italy., Pederzolli C; Fondazione Bruno Kessler, Sensors and Devices Center, Via Sommarive 18, 38123, Trento, Italy., Guardiani C; FTH srl, Via Sommarive 18, 38123, Trento, Italy., Pasquardini L; Indivenire srl, Via Sommarive 18, 38123, Trento, Italy; Department of Engineering, University of Campania 'Luigi Vanvitelli', Via Roma 29, 81031, Aversa, Italy. Electronic address: l.pasquardini@indiveni.re.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2025 Jan 01; Vol. 282, pp. 127035. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1016/j.talanta.2024.127035
Abstrakt: In the recent years, the number of Point-Of-Care-Tests (POCTs) available for clinical diagnostic has steadily increased. POCTs provide a near-patient testing with the potential to generate a result quickly so that appropriate treatment can be implemented, leading to improved clinical outcomes compared to traditional laboratory testing. Technological advances, such as miniaturization of sensors and improved instrumentation, have revolutionized POCTs, enabling the development of smaller and more accurate devices. In this context, it has also gained increasing importance the screening of various analytes simultaneously to increase specificity and improve the characterization of the disease. This study is aimed at developing and characterizing a photonic integrated circuit for multiple markers detection, which represents the functional core towards a full developed POCT device for clinical pathology applications. The photonic sensor, based on microring resonators (MRRs), is functionalized by immobilizing specific antibodies on a copolymer layer deposited on the MRR's surfaces. Surface chemical techniques were employed to analyse the surface chemical characteristics while fluorescence microscopy was involved to analyse the resulting bioreceptor surface density. The photonic sensor is characterized for the parallel detection of two biomarkers, the C-Reactive Protein (CRP) and the Creatine-Kinase-MB (CK-MB). The analyte-antibody binding curves were obtained both in buffer and in filtered un-diluted artificial saliva showing promising results both in terms of sensitivity, with limit of detection (LOD) of 103 pM for CRP and 140 pM for CK-MB, and in terms of specificity. These encouraging results let the assembly of a highly sensitive POC device for molecular diagnostics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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