| Autor: |
Hodgson H; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K., Stephenson MJ; School of Chemistry, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K., Kikuchi S; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K., Martin LBB; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K., Liu JCT; Department of Chemistry, Stanford University, Stanford, California 94305, United States., Casson R; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K., Rejzek M; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K., Sattely ES; Department of Chemical Engineering, Stanford University, Stanford, California 94305, United States.; Howard Hughes Medical Institute, Stanford University, Stanford, California 94305, United States., Osbourn A; Department of Biochemistry and Metabolism, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, U.K. |
| Abstrakt: |
The furan ring is a defining feature of limonoids, a class of highly rearranged and bioactive plant tetranortriterpenoids. We recently reported an apparent complete biosynthetic pathway to these important natural furanoids. Herein, we disclose the subsequent discovery of a yield-boosting "missing link" carboxylesterase that selectively deprotects a late-stage intermediate, so triggering more efficient furan biosynthesis. This has allowed, for the first time, the isolation and structural elucidation of unknown intermediates, refining our understanding of furan formation in limonoid biosynthesis. |
