Detection and transport of environmental DNA from two federally endangered mussels.

Autor: Sansom BJ; U.S. Geological Survey-Columbia Environmental Research Center, Columbia, MO, United States of America., Ruiz-Ramos DV; U.S. Geological Survey-Columbia Environmental Research Center, Columbia, MO, United States of America.; Department of Natural Sciences, University of Maryland Eastern Shore, Princess Anne, MD, United States of America., Thompson NL; U.S. Geological Survey-Columbia Environmental Research Center, Columbia, MO, United States of America., Roberts MO; U.S. Geological Survey-Columbia Environmental Research Center, Columbia, MO, United States of America., Taylor ZA; Maryland Department of Natural Resources, Annapolis, MD, United States of America.; Department of Fish and Wildlife Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States of America., Ortiz K; Department of Fish and Wildlife Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States of America., Jones JW; Department of Fish and Wildlife Conservation, U.S. Fish and Wildlife Service, Blacksburg, VA, United States of America., Richter CA; U.S. Geological Survey-Columbia Environmental Research Center, Columbia, MO, United States of America., Klymus KE; U.S. Geological Survey-Columbia Environmental Research Center, Columbia, MO, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Oct 17; Vol. 19 (10), pp. e0304323. Date of Electronic Publication: 2024 Oct 17 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0304323
Abstrakt: Environmental DNA (eDNA) offers a novel approach to supplement traditional surveys and provide increased spatial and temporal information on species detection, and it can be especially beneficial for detecting at risk or threatened species with minimal impact on the target species. The transport of eDNA in lotic environments is an important component in providing more informed descriptions of where and when a species is present, but eDNA transport phenomena are not well understood. In this study, we used species-specific assays to detect eDNA from two federally endangered mussels in two geographically distinct rivers. Using the eDNA concentrations measured from field samples, we developed a one-dimensional (1D) hydrodynamic transport model to predict the downstream fate and transport of eDNA. We detected eDNA from both federally endangered mussels across several seasons and flow rates and up to 3.5 km downstream from the source populations, but the detection rates and eDNA concentrations were highly variable across and within rivers and study reaches. Our 1D transport models successfully integrated the variability of the eDNA field samples into the model predictions and overall model results were generally within ±1 standard error of the eDNA field concentration values. Overall, the results of this study demonstrate the importance of optimizing the spatial locations from where eDNA is collected downstream from a source population, and it highlights the need to improve understanding on the shedding mechanisms and magnitude of eDNA from source populations and biogeomorphic processes that influence eDNA transport.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
Databáze: MEDLINE
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