Comparative analysis of porcine-uterine decellularization for bioactive-molecule preservation and DNA removal.

Autor:	Fazel Anvari Yazdi A; Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada., Tahermanesh K; Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran., Ejlali M; College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada., Babaei-Ghazvini A; Department of Chemical and Biological Engineering, University of Saskatchewan, Saskatoon, SK, Canada., Acharya B; Department of Chemical and Biological Engineering, University of Saskatchewan, Saskatoon, SK, Canada., Badea I; College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada., MacPhee DJ; Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada., Chen X; Division of Biomedical Engineering, University of Saskatchewan, Saskatoon, Canada.; Department of Mechanical Engineering, University of Saskatchewan, Saskatoon, SK, Canada.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Oct 02; Vol. 12, pp. 1418034. Date of Electronic Publication: 2024 Oct 02 (Print Publication: 2024).
DOI:	10.3389/fbioe.2024.1418034
Abstrakt:	Introduction: Decellularized uterine extracellular matrix has emerged as a pivotal focus in the realm of biomaterials, offering a promising source in uterine tissue regeneration, research on disease diagnosis and treatments, and ultimately uterine transplantation. In this study, we examined various protocols for decellularizing porcine uterine tissues, aimed to unravel the intricate dynamics of DNA removal, bioactive molecules preservation, and microstructural alterations. Methods: Porcine uterine tissues were treated with 6 different, yet rigorously selected and designed, protocols with sodium dodecyl sulfate (SDS), Triton ® X-100, peracetic acid + ethanol, and DNase I. After decellularization, we examined DNA quantification, histological staining (H&E and DAPI), glycosaminoglycans (GAG) assay, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and Thermogravimetric Analysis (TGA). Results: A comparative analysis among all 6 protocols was conducted with the results demonstrating that all protocols achieved decellularization; while 0.1% SDS + 1% Triton ® X-100, coupled with agitation, demonstrated the highest efficiency in DNA removal. Also, it was found that DNase I played a key role in enhancing the efficiency of the decellularization process by underscoring its significance in digesting cellular contents and eliminating cell debris by 99.79% (19.63 ± 3.92 ng/mg dry weight). Conclusions: Our findings enhance the nuanced understanding of DNA removal, GAG preservation, microstructural alteration, and protein decomposition in decellularized uterine extracellular matrix, while highlighting the importance of decellularization protocols designed for intended applications. This study along with our findings represents meaningful progress for advancing the field of uterine transplantation and related tissue engineering/regenerative medicine. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Fazel Anvari Yazdi, Tahermanesh, Ejlali, Babaei-Ghazvini, Acharya, Badea, MacPhee and Chen.)
Databáze:	MEDLINE
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