Vitamin B 12 protects necrosis of acinar cells in pancreatic tissues with acute pancreatitis.

Autor: Chen Y; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Li X; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Lu R; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China.; Department of Occupational and Environmental Health West China School of Public Health and West China Fourth Hospital Sichuan University Chengdu China.; West China-PUMC C.C. Chen Institute of Health, West China School of Public Health and West China Fourth Hospital, Sichuan University Chengdu China., Lv Y; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Wu Y; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Ye J; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Zhao J; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Li L; School of Basic Medicine Southwest Medical University Luzhou China., Huang Q; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Meng W; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Long F; Department of Gastrointestinal Bariatric, and Metabolic Surgery Research Center for Nutrition Metabolism & Food Safety West China-PUMC C.C. Chen Institute of Health West China School of Public Health and West China Fourth Hospital, Sichuan University Chengdu China., Huang W; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Xia Q; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China., Yu J; Longgang Central Hospital Shenzhen China., Fan C; Department of Gastrointestinal Bariatric, and Metabolic Surgery Research Center for Nutrition Metabolism & Food Safety West China-PUMC C.C. Chen Institute of Health West China School of Public Health and West China Fourth Hospital, Sichuan University Chengdu China.; Department of Oncology and Department of Biomedical and Clinical Sciences Linköping University Linköping Sweden., Mo X; West China Center of Excellence for Pancreatitis Institute of Integrated Traditional Chinese and Western Medicine Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu China.
Jazyk: angličtina
Zdroj: MedComm [MedComm (2020)] 2024 Oct 15; Vol. 5 (11), pp. e686. Date of Electronic Publication: 2024 Oct 15 (Print Publication: 2024).
DOI: 10.1002/mco2.686
Abstrakt: Pharmacological agents regarding the most optimal treatments of acute pancreatitis remain. One-carbon metabolism nutrients as therapeutic agents in many diseases might be involved in acute pancreatitis. The roles are acquired exploration in acute pancreatitis. We utilized Mendelian randomization to assess the causal impact of folate, homocysteine, and vitamin B 12 (VB 12 ) on acute pancreatitis. Wild-type and corresponding genetically modified mouse models were used to verify the genetic correlating findings. A negative association between genetically predicted serum VB 12 levels and risks of acute pancreatitis was identified in human population. The transcobalamin receptor (TCblR)/ CD320 gene ablation that decreased cellular VB 12 uptake and ATP production in pancreatic tissues promoted necrosis, resulting in much severe pathological changes of induced acute pancreatitis in mice. VB 12 pretreatment and posttreatment dramatically increased ATP levels in pancreatic tissues and reduced the necrosis, then the elevated levels of amylase in serum, the levels of CK-19, the activity of trypsin, and T lymphocyte infiltration in pancreatic tissues, prevented the pancreatic gross loss and ameliorated histopathological changes of mouse pancreases with induced acute pancreatitis. The results reveal that VB 12 is potential as a therapeutic agent to inhibit tissue injuries and adaptive inflammatory responses in the pancreas in patients with acute pancreatitis.
Competing Interests: The authors declare they have no conflicts of interest.
(© 2024 The Author(s). MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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