| Autor: |
Paterson TE; Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. g.reilly@sheffield.ac.uk.; School of Clinical Dentistry, University of Sheffield, Sheffield, UK.; INSIGNEO Institute for in silico Medicine, University of Sheffield, Sheffield, UK., Owen R; School of Pharmacy, University of Nottingham Biodiscovery Institute, University of Nottingham, Nottingham, UK. robert.owen@nottingham.ac.uk., Sherborne C; Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. g.reilly@sheffield.ac.uk., Bahmaee H; Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. g.reilly@sheffield.ac.uk.; INSIGNEO Institute for in silico Medicine, University of Sheffield, Sheffield, UK., Harding AL; School of Clinical Dentistry, University of Sheffield, Sheffield, UK., Green NH; Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. g.reilly@sheffield.ac.uk.; INSIGNEO Institute for in silico Medicine, University of Sheffield, Sheffield, UK., Reilly GC; Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. g.reilly@sheffield.ac.uk.; INSIGNEO Institute for in silico Medicine, University of Sheffield, Sheffield, UK., Claeyssens F; Department of Materials Science and Engineering, The Kroto Research Institute, University of Sheffield, Sheffield, UK. g.reilly@sheffield.ac.uk. |
| Abstrakt: |
Improving our ability to treat skeletal defects is a critical medical challenge that necessitates the development of new biomaterials. One promising approach involves the use of degradable polymer microparticles with an interconnected internal porosity. Here, we employed a double emulsion to generate such round microparticles (also known as microspheres) from a polycaprolactone-based polymerised high internal phase emulsion (polyHIPE). These microspheres effectively supported the growth of mesenchymal progenitors over a 30-day period, and when maintained in osteogenic media, cells deposited a bone-like extracellular matrix, as determined by histological staining for calcium and collagen. Interestingly, cells with an osteocyte-like morphology were observed within the core of the microspheres indicating the role of a physical environment comparable to native bone for this phenotype to occur. At later timepoints, these cultures had significantly increased mRNA expression of the osteocyte-specific markers dentin matrix phosphoprotein-1 (Dmp-1) and sclerostin, with sclerostin also observed at the protein level. Cells pre-cultured on porous microspheres exhibited enhanced survival rates compared to those pre-cultured on non-porous counterparts when injected. Cells precultured on both porous and non-porous microspheres promoted angiogenesis in a chorioallantoic membrane (CAM) assay. In summary, the polycaprolactone polyHIPE microspheres developed in this study exhibit significant promise as an alternative to traditional synthetic bone graft substitutes, offering a conducive environment for cell growth and differentiation, with the potential for better clinical outcomes in bone repair and regeneration. |
