CHUP1 restricts chloroplast movement and effector-triggered immunity in epidermal cells.

Autor: Nedo AO; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.; Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19713, USA.; Department of Biological Sciences, University of Delaware, Newark, DE, 19716, USA., Liang H; Department of Computer & Information Sciences, University of Delaware, Newark, DE, 19716, USA., Sriram J; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.; Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19713, USA., Razzak MA; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.; Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19713, USA., Lee JY; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.; Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19713, USA.; Department of Biological Sciences, University of Delaware, Newark, DE, 19716, USA., Kambhamettu C; Department of Computer & Information Sciences, University of Delaware, Newark, DE, 19716, USA., Dinesh-Kumar SP; Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, CA, 95616, USA., Caplan JL; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA.; Delaware Biotechnology Institute, University of Delaware, Newark, DE, 19713, USA.; Department of Biological Sciences, University of Delaware, Newark, DE, 19716, USA.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Dec; Vol. 244 (5), pp. 1864-1881. Date of Electronic Publication: 2024 Oct 17.
DOI: 10.1111/nph.20147
Abstrakt: Chloroplast Unusual Positioning 1 (CHUP1) plays an important role in the chloroplast avoidance and accumulation responses in mesophyll cells. In epidermal cells, prior research showed silencing CHUP1-induced chloroplast stromules and amplified effector-triggered immunity (ETI); however, the underlying mechanisms remain largely unknown. CHUP1 has a dual function in anchoring chloroplasts and recruiting chloroplast-associated actin (cp-actin) filaments for blue light-induced movement. To determine which function is critical for ETI, we developed an approach to quantify chloroplast anchoring and movement in epidermal cells. Our data show that silencing NbCHUP1 in Nicotiana benthamiana plants increased epidermal chloroplast de-anchoring and basal movement but did not fully disrupt blue light-induced chloroplast movement. Silencing NbCHUP1 auto-activated epidermal chloroplast defense (ECD) responses including stromule formation, perinuclear chloroplast clustering, the epidermal chloroplast response (ECR), and the chloroplast reactive oxygen species (ROS), hydrogen peroxide (H 2 O 2 ). These findings show chloroplast anchoring restricts a multifaceted ECD response. Our results also show that the accumulated chloroplastic H 2 O 2 in NbCHUP1-silenced plants was not required for the increased basal epidermal chloroplast movement but was essential for increased stromules and enhanced ETI. This finding indicates that chloroplast de-anchoring and H 2 O 2 play separate but essential roles during ETI.
(© 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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