Cleistocalyx nervosum var. paniala mitigates oxidative stress and inflammation induced by PM 10 soluble extract in trophoblast cells via miR-146a-5p.

Autor: Chaiwangyen W; Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand. wittaya.ch@up.ac.th., Khantamat O; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand., Pintha K; Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand., Kangwan N; Division of Physiology, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand., Onsa-Ard A; Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand., Nuntaboon P; Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand., Songkrao A; Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand., Thippraphan P; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand., Chaiyasit D; Clinical Chemistry Laboratory, Chiang Rai Prachanukroh Hospital, Chiang Rai, 57000, Thailand., de Sousa FLP; Department of Gynecology and Obstetrics, UNILUS (Centro Universitário Lusíada), Santos, 11050-071, Brazil.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 16; Vol. 14 (1), pp. 24265. Date of Electronic Publication: 2024 Oct 16.
DOI: 10.1038/s41598-024-73000-y
Abstrakt: Air pollution poses a significant global concern, notably impacting pregnancy outcomes through mechanisms such as DNA damage, oxidative stress, inflammation, and altered miRNA expression, all of which can adversely affect trophoblast functions. Cleistocalyx nervosum var. paniala, known for its abundance of anthocyanins with diverse biological activities including anti-mutagenic, antioxidant, and anti-inflammatory properties, is the focus of this study examining its effect on Particulate Matter 10 (PM 10 ) soluble extract-induced trophoblast cell dysfunction via miRNA expression. The study involved the extraction of C. nervosum fruit using 70% ethanol, followed by fractionation with hexane, dichloromethane, and ethyl acetate. Subsequent testing for total phenolics, flavonoids, anthocyanins, and antioxidant activity revealed the ethyl acetate fraction (CN-EtOAcF) as possessing the highest phenolic and anthocyanin content along with potent antioxidant activity, prompting its selection for further investigation. In vitro studies on HTR-8/SVneo cells demonstrated that 5-10 µg/mL PM 10 soluble extract exposure inhibited cell proliferation, migration, invasion, and induced apoptosis. However, pretreatment with 20-80 µg/mL CN-EtOAcF followed by 5 µg/mL PM 10 soluble extract exposure exhibited protective effects against PM 10 soluble extract-induced damage, including inflammation inhibition and intracellular ROS suppression. Notably, CN-EtOAcF down-regulated PM 10 -induced miR-146a-5p expression, with SOX5 identified as a potential target. Overall, CN-EtOAcF demonstrated the potential to protect against PM 10 -induced harm in trophoblast cells, suggesting its possible application in future therapeutic approaches.
(© 2024. The Author(s).)
Databáze: MEDLINE
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