Spatial transcriptomic analysis of adult hippocampal neurogenesis in the human brain.

Autor:	Simard S; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Rahimian R; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Davoli MA; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Théberge S; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Matosin N; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Turecki G; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Nagy C; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar)., Mechawar N; From the McGill Group for Suicide Studies, Douglas Mental Health University Institute, McGill University, Montréal, Que. (Simard, Rahimian, Antonietta, Théberge, Turecki, Nagy, Mechawar); the School of Medical Sciences, Faculty of Medicine and Health, Charles Perkins Centre, The University of Sydney, Camperdown, Australia (Matosin); the Department of Psychiatry, McGill University, Montréal, Que. (Turecki, Nagy, Mechawar). naguib.mechawar@mcgill.ca.
Jazyk:	angličtina
Zdroj:	Journal of psychiatry & neuroscience : JPN [J Psychiatry Neurosci] 2024 Oct 16; Vol. 49 (5), pp. E319-E333. Date of Electronic Publication: 2024 Oct 16 (Print Publication: 2024).
DOI:	10.1503/jpn.240026
Abstrakt:	Background: Adult hippocampal neurogenesis has been extensively characterized in rodent models, but its existence in humans remains controversial. We sought to assess the phenomenon in postmortem human hippocampal samples by combining spatial transcriptomics and multiplexed fluorescent in situ hybridization. Methods: We computationally examined the spatial expression of various canonical neurogenesis markers in postmortem dentate gyrus (DG) sections from young and middle-aged sudden-death males. We conducted in situ assessment of markers expressed in neural stem cells, proliferative cells, and immature granule neurons in postmortem DG sections from infant, adolescent, and middle-aged males. Results: We examined frozen DG tissue from infant ( n = 1, age 2 yr), adolescent ( n = 1, age 16 yr), young adult ( n = 2, mean age 23.5 yr), and middle-aged ( n = 2, mean age 42.5 yr) males, and frozen-fixed DG tissue from middle-aged males ( n = 6, mean age 43.5 yr). We detected very few cells expressing neural stem cell and proliferative markers in the human DG from childhood to middle age. However, at all ages, we observed a substantial number of DG cells expressing the immature neuronal marker DCX . Most DCX + cells displayed an inhibitory phenotype, while the remainder were non-committed or excitatory in nature. Limitations: The study was limited by small sample sizes and included samples only from males. Conclusion: Our findings indicate very low levels of hippocampal neurogenesis throughout life and the existence of a local reserve of plasticity in the adult human hippocampus. Overall, our study provides important insight into the distribution and phenotype of cells expressing neurogenesis markers in the adult human hippocampus. Competing Interests: Competing interests:: None declared. (© 2024 CMA Impact Inc. or its licensors.)
Databáze:	MEDLINE
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