TRICHINELLA MURRELLI POZIO AND LA ROSA, 2000 IN A GRAY FOX (UROCYON CINEREOARGENTEUS) FROM PENNSYLVANIA: A NEW HOST RECORD FOR THIS ZOONOTIC NEMATODE.

Autor:	Thompson PC; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Araujo LS; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Gupta A; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Kay S; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Kwok OCH; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Battle J; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Van Why K; United States Department of Agriculture Wildlife Services, PO Box 60827, Harrisburg, Pennsylvania 17106., Brown JD; Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania 16802., Rosenthal BM; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350., Dubey JP; United States Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705-2350.
Jazyk:	angličtina
Zdroj:	The Journal of parasitology [J Parasitol] 2024 Sep 01; Vol. 110 (5), pp. 502-505.
DOI:	10.1645/24-53
Abstrakt:	Trichinella murrelli Pozio and La Rosa, 2000, is the primary sylvatic trichinellid encountered in temperate North America. During a survey for Sarcocystis in wild canids, a single worm matching the morphology of encapsulated Trichinella was observed in a muscle tissue squash from a gray fox male originating from Pennsylvania. The worm was photographed and then separated from the host tissue by artificial digestion, and genomic DNA was extracted from the worm. This DNA was subjected to species-specific multiplex PCR and short-read genomic sequencing. The banding pattern of the multiplex PCR indicated that the worm was T. murrelli, and the sequence of the mitochondrial Cytochrome c oxidase 1 gene and the ribosomal 18S ribosomal RNA, Internal Transcribed Spacer 1, 5.8S ribosomal RNA, Internal Transcribed Spacer 2, and 28S ribosomal RNA confirmed the diagnosis. This is the first report of T. murrelli in gray foxes that includes assays for assigning parasite species. This report confirms suspected data from surveys conducted over 30 yr ago and establishes a new host record for T. murrelli. (© American Society of Parasitologists 2024.)
Databáze:	MEDLINE
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