Ion chromatography coupled with optical emission spectrometry (IC-ICP-OES) methodology for the analysis of inositol phosphates in food and feed.

Autor: Henninger C; Hochschule Offenburg, Fakultät Maschinenbau und Verfahrenstechnik, Badstraße 24, 77652 Offenburg, Germany; Karlsruhe Institute of Technology (KIT), Department of Chemical and Process Engineering, Kaiserstraße 12, 76131 Karlsruhe, Germany., Stadelmann T; Picea Biosolutions GmbH, Carl-Benz-Straße 30, 77797 Ohlsbach, Germany., Heid D; Picea Biosolutions GmbH, Carl-Benz-Straße 30, 77797 Ohlsbach, Germany., Ochsenreither K; Karlsruhe Institute of Technology (KIT), Department of Chemical and Process Engineering, Kaiserstraße 12, 76131 Karlsruhe, Germany., Eisele T; Hochschule Offenburg, Fakultät Maschinenbau und Verfahrenstechnik, Badstraße 24, 77652 Offenburg, Germany. Electronic address: thomas.eisele@hs-offenburg.de.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2025 Jan 15; Vol. 463 (Pt 4), pp. 141437. Date of Electronic Publication: 2024 Sep 28.
DOI: 10.1016/j.foodchem.2024.141437
Abstrakt: This study presents the development of ion chromatography coupled with inductively coupled plasma optical emission spectrometry (IC-ICP-OES) for the simultaneous determination and quantification of inositol phosphates (InsP x ). Using a CarboPac PA100 column with a nitric acid-water gradient, 28 InsP x isomers (InsP 6 to InsP 2 ) were separated within 33 min. The method eliminates baseline drift and post-column derivatization thereby simplifying detection and quantification. It achieves low detection limits of 63 μg/L P across a range of 63-3200 μg/L P. Various extraction and sample preparation methods for food and feed matrices were tested, including acidic and alkaline agents, C 18 SPE and spin concentrators. The analysis shows intra-day and intra-laboratory reproducibility with deviations smaller than 1 % for standard solutions and under 4 % for feed samples (80 % recovery rate of phytate). This methodology is applicable to explore enzymatic degradation pathways and the analysis of InsP x in complex food and animal feed matrices.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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