| Autor: |
Thome CP; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80303, USA., Fowle JP; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80303, USA., McDonnell P; Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA., Zultak J; Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA., Jayaram K; Paul M. Rady Department of Mechanical Engineering, University of Colorado Boulder, Boulder, CO 80309, USA.; Biomedical Engineering Program, University of Colorado Boulder, Boulder, CO 80303, USA., Neumann AK; Department of Pathology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA., López GP; Department of Chemical and Biological Engineering, University of New Mexico, Albuquerque, NM 87131, USA., Shields CW 4th; Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO 80303, USA.; Biomedical Engineering Program, University of Colorado Boulder, Boulder, CO 80303, USA. |
| Abstrakt: |
Most biosensing techniques require complex processing steps that generate prolonged workflows and introduce potential points of error. Here, we report an acoustic pipette to purify and label biomarkers in 70 minutes. A key aspect of this technology is the use of functional negative acoustic contrast particles (fNACPs), which display biorecognition motifs for the specific capture of biomarkers from whole blood. Because of their large size and compressibility, the fNACPs robustly trap along the pressure antinodes of a standing wave and separate from blood components in under 60 seconds with >99% efficiency. fNACPs are subsequently fluorescently labeled in the pipette and are analyzed by both a custom, portable fluorimeter and flow cytometer. We demonstrate the detection of anti-ovalbumin antibodies from blood at picomolar levels (35 to 60 pM) with integrated controls showing minimal nonspecific adsorption. Overall, this system offers a simple and versatile approach for the rapid, sensitive, and specific capture of biomolecules. |
