A plant-based oligomeric CD2v extracellular domain antigen exhibits equivalent immunogenicity to the live attenuated vaccine ASFV-G-∆I177L.

Autor: Nguyen GT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Le TT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Vu SDT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Nguyen TT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Le MTT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Pham VT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Nguyen HTT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Ho TT; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Hoang HTT; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Tran HX; National Veterinary Joint Stock Company - NAVETCO, Ho Chi Minh City, Viet Nam., Chu HH; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam.; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam., Pham NB; Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam. pbngoc@ibt.ac.vn.; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Viet Nam. pbngoc@ibt.ac.vn.
Jazyk: angličtina
Zdroj: Medical microbiology and immunology [Med Microbiol Immunol] 2024 Oct 16; Vol. 213 (1), pp. 22. Date of Electronic Publication: 2024 Oct 16.
DOI: 10.1007/s00430-024-00804-0
Abstrakt: African swine fever (ASF), caused by the African swine fever virus (ASFV), is a deadly, highly contagious disease in both domestic pigs and wild boar. With mortality up to 100%, the disease has been making a serious impact on the swine industry worldwide. Because no effective antiviral treatment has been observed, proactive prevention such as vaccination remains the key to controlling the outbreak. In the pursuit of expediting vaccine development, our current work has made the first report for heterologous production of the viral outer envelope glycoprotein CD2v extracellular domain (CD2v ED), a proposed promising vaccine antigen candidate in the "green" synthetic host Nicotiana benthamiana. Protein oligomerization strategies were implemented to increase the immunogenicity of the target antigen. Herein, the protein was expressed in oligomeric forms based on the C-terminally fused GCN4pII trimerization motif and GCN4pII_TP oligomerization motif. Quantitative western blot analysis showed significantly higher expression of trimeric CD2v ED_GCN4pII with a yield of about 12 mg/100 g of fresh weight, in comparison to oligomeric CD2v ED_GCN4pII_TP, revealing the former is the better choice for further studies. The results of purification and size determination by size exclusion chromatography (SEC) illustrated that CD2v ED_GCN4pII was successfully produced in stable oligomeric forms throughout the extraction, purification, and analysis process. Most importantly, purified CD2v ED_GCN4pII was demonstrated to induce both humoral and cellular immunity responses in mice to extents equivalent to those of the live attenuated vaccine ASFV-G-∆I177L, suggesting it as the potential subunit vaccine candidate for preventing ASFV.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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