Human Papilloma Virus (HPV) Detection in Oral Rinse vs. Oral Sponge: A Preliminary Accuracy Report in Oral Cancer Patients.

Autor: Panzarella V; Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, 90127 Palermo, Italy.; Center for Sustainability and Ecological Transition (CSTE), University of Palermo, 90133 Palermo, Italy., Buttà M; Department of Health Promotion, Mother and Childcare, Internal Medicine and Medical Specialties (ProMISE), University of Palermo, 90127 Palermo, Italy., Buttacavoli F; Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, 90127 Palermo, Italy.; Unit of Oral Medicine and Dentistry for Fragile Patients, Department of Rehabilitation, Fragility, and Continuity of Care, University Hospital 'Policlinico Paolo Giaccone' in Palermo, 90127 Palermo, Italy., Capra G; Department of Health Promotion, Mother and Childcare, Internal Medicine and Medical Specialties (ProMISE), University of Palermo, 90127 Palermo, Italy., Firenze A; Center for Sustainability and Ecological Transition (CSTE), University of Palermo, 90133 Palermo, Italy.; Department of Health Promotion, Mother and Childcare, Internal Medicine and Medical Specialties (ProMISE), University of Palermo, 90127 Palermo, Italy., Serra N; Department of Neuroscience, Reproductive Sciences and Dentistry, Audiology Section University Federico II of Naples, 80131 Napoli, Italy., Giuliana G; Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, 90127 Palermo, Italy., Pizzo G; Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, 90127 Palermo, Italy.; Unit of Oral Medicine and Dentistry for Fragile Patients, Department of Rehabilitation, Fragility, and Continuity of Care, University Hospital 'Policlinico Paolo Giaccone' in Palermo, 90127 Palermo, Italy., Campisi G; Unit of Oral Medicine and Dentistry for Fragile Patients, Department of Rehabilitation, Fragility, and Continuity of Care, University Hospital 'Policlinico Paolo Giaccone' in Palermo, 90127 Palermo, Italy., Mauceri R; Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, 90127 Palermo, Italy.; Unit of Oral Medicine and Dentistry for Fragile Patients, Department of Rehabilitation, Fragility, and Continuity of Care, University Hospital 'Policlinico Paolo Giaccone' in Palermo, 90127 Palermo, Italy.
Jazyk: angličtina
Zdroj: Cancers [Cancers (Basel)] 2024 Sep 24; Vol. 16 (19). Date of Electronic Publication: 2024 Sep 24.
DOI: 10.3390/cancers16193256
Abstrakt: Background/objectives: Human Papillomavirus (HPV) is a significant etiological factor in the development of oropharyngeal carcinogenesis. The detection of HPV in oral squamous cell carcinoma (OSCC) could be also crucial for diagnosis, prognosis, and treatment planning. This study compares the efficacy and accuracy of two non-invasive sampling methods, oral rinse, and oral sponge, in detecting HPV DNA in patients with OSCC.
Methods: Twenty-six patients with histologically confirmed OSCCs were recruited (M/F = 15/11; mean age 68.6). From each patient, two self-collected oral specimens, in the form of an oral rinse and a salivary sponge (i.e., LolliSponge), were collected, and subsequently processed, utilizing INNO-LiPA HPV Genotyping Extra II for HPV DNA detection; Results: Oral sponge detection showed high specificity (100%), sensitivity (85.7%), and accuracy (96.2%) compared to the oral rinse sampling, also demonstrating an area AUC for its diagnostic performance significantly greater than 0.5 (0.93 vs. 0.5, p < 0.0001).
Conclusions: This study supports that oral sponge sampling offers valuable non-invasive alternatives for HPV detection in patients with OSCC, with the potentiality to facilitate saliva sampling in patients that may exhibit functional deficit due to OSCC. Further research is recommended to validate these findings in larger cohorts and to explore the integration of these methods into routine clinical practice for the management of HPV-related OSCC.
Databáze: MEDLINE
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