Genomic Instability in the Lymphocytes of Dogs with Squamous Cell Carcinoma.

Autor: Wójcik E; Institute of Animal Science and Fisheries, University of Siedlce, 08-110 Siedlce, Poland., Kot E; Institute of Animal Science and Fisheries, University of Siedlce, 08-110 Siedlce, Poland., Wójcik I; Institute of Animal Science and Fisheries, University of Siedlce, 08-110 Siedlce, Poland., Wysokińska A; Institute of Animal Science and Fisheries, University of Siedlce, 08-110 Siedlce, Poland., Matusevičius P; Department of Animal Nutrition, Lithuanian University of Health Sciences, LT-47181 Kaunas, Lithuania.
Jazyk: angličtina
Zdroj: Animals : an open access journal from MDPI [Animals (Basel)] 2024 Sep 24; Vol. 14 (19). Date of Electronic Publication: 2024 Sep 24.
DOI: 10.3390/ani14192754
Abstrakt: Genome instability is a characteristic trait of tumours and includes changes in DNA and in chromosomes. The aim of the study was to identify chromosome damage using the sister chromatid exchange assay and DNA fragmentation by the comet assay in dogs with cancer, as well as to determine the suitability of these techniques for the assessment of chromatin stability in healthy and sick dogs. The assays identified genomic instabilities in dogs with cancer (squamous cell carcinoma) and in healthy dogs. The genetic assays are very sensitive and can be used as biomarkers of normal DNA replication and repair potential and the maintenance of control over the entire cell cycle. The use of the cytogenetic tests will enable the more precise assessment of genome stability and integrity in animals and make it possible to determine the number of chromosomal instabilities generated in a given individual, which can be indicative of its health status. The identification of instabilities can be used in routine diagnostic examination in dogs with cancer for more accurate diagnosis and prognosis.
Databáze: MEDLINE
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