| Autor: |
Rodríguez-Valdovinos KY; Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Michoacan, Mexico., Salgado-Garciglia R; Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Michoacan, Mexico., Hernández-García A; Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Michoacan, Mexico., Saavedra-Molina A; Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Michoacan, Mexico., Del Río-Torres REN; Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Michoacan, Mexico., López-Meza JE; Centro Multidisciplinario de Estudios en Biotecnología-FMVZ, Universidad Michoacana de San Nicolás de Hidalgo, Tarímbaro 58880, Michoacan, Mexico., Monribot-Villanueva JL; Red de Estudios Moleculares Avanzados, Clúster BioMimic®, Instituto de Ecología, A.C., Xalapa 91073, Veracruz, Mexico., Guerrero-Analco JA; Red de Estudios Moleculares Avanzados, Clúster BioMimic®, Instituto de Ecología, A.C., Xalapa 91073, Veracruz, Mexico., Medina-Medrano JR; Licenciatura en Genómica Alimentaria, Universidad de La Ciénega del Estado de Michoacán de Ocampo, Sahuayo 59103, Michoacan, Mexico. |
| Abstrakt: |
The Verbesina gender represents the second most diverse group from the Asteraceae family in Mexico; Verbesina sphaerocephala is one of the most distributed species along the Mexican territory. This species has been poorly studied, reporting the presence of some bioactive compounds with antioxidant and antibacterial activity. In this study, phenolic and flavonoid contents and composition, antioxidant and antifungal activities of aqueous extracts of the stem of V. sphaerocephala and its fractions were determined. The results showed that the highest antifungal activity against Botrytis cinerea was shown by the aqueous extract (IC 50 : 0.10 mg/mL) and the ethyl acetate fraction (IC 50 : 14.8 mg/mL). In addition, the aqueous extract and the ethyl acetate fraction exhibited the highest phenolic (21.40 and 21.26 mg gallic acid equivalents per gram of dry extract, respectively) and flavonoid contents (11.53 and 3.71 mg rutin equivalents, respectively) and high antioxidant activity determined by the Total Antioxidant Capacity (20.62 and 40.21 mg ascorbic acid equivalents per gram of dry extract, respectively), Ferric Reducing Power (74.76 and 129.57 mg gallic acid equivalents per gram of dry extract, respectively), DPPH (IC 50 : 12.38 and 7.36 mg/mL, respectively), and ABTS (IC 50 : 5.60 and 7.76 mg/mL, respectively) methods. Twelve phenolic compounds were detected in the aqueous extract using UPLC-MS analysis, of which the major ones were protocatechuic, vanillic, and hydroxybenzoic acid, while in the ethyl acetate fraction, the presence of 18 phenolic compounds were identified, of which the majority were vanillin, rutin, and hydroxybenzoic acid. The results of this research demonstrate that the aqueous extract of V. sphaerocephala stems has phenolic compounds with antifungal and antioxidant activity. |
