The Immobilization of an FGF2-Derived Peptide on Culture Plates Improves the Production and Therapeutic Potential of Extracellular Vesicles from Wharton's Jelly Mesenchymal Stem Cells.

Autor: Lee Y; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea., Lim KM; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.; R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea., Bong H; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea., Lee SB; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea., Jeon TI; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea., Lee SY; New Materials R&D Center of AMOGREANTECH Co., Ltd., 609-1 Wolha-ro, Haseong-myeon, Gimpo-si 10011, Republic of Korea., Park HS; New Materials R&D Center of AMOGREANTECH Co., Ltd., 609-1 Wolha-ro, Haseong-myeon, Gimpo-si 10011, Republic of Korea., Kim JY; New Materials R&D Center of AMOGREANTECH Co., Ltd., 609-1 Wolha-ro, Haseong-myeon, Gimpo-si 10011, Republic of Korea., Song K; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.; R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea., Kang GH; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.; R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea., Kim SJ; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.; R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea., Song M; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.; R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea., Cho SG; Department of Stem Cell and Regenerative Biotechnology, Molecular & Cellular Reprogramming Center and Institute of Advanced Regenerative Science, Konkuk University, Seoul 05029, Republic of Korea.; R&D Team, StemExOne Co., Ltd., 307, KU Technology Innovation Building, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Oct 04; Vol. 25 (19). Date of Electronic Publication: 2024 Oct 04.
DOI: 10.3390/ijms251910709
Abstrakt: The skin is an essential organ that protects the body from external aggressions; therefore, damage from various wounds can significantly impair its function, and effective methods for regenerating and restoring its barrier function are crucial. This study aimed to mass-produce wound-healing exosomes using a fragment of the fibroblast growth factor 2 (FGF2)-derived peptide (FP2) to enhance cell proliferation and exosome production. Our experiments demonstrated increased cell proliferation when Wharton's jelly mesenchymal stem cells (WJ MSCs) were coated with FP2. Exosomes from FP2-coated WJ MSCs were analyzed using nanoparticle-tracking analysis, transmission electron microscopy, and Western blotting. Subsequently, fibroblasts were treated with these exosomes, and their viability and migration effects were compared. Anti-inflammatory effects were also evaluated by inducing pro-inflammatory factors in RAW264.7 cells. The treatment of fibroblasts with FP2-coated WJ MSC-derived exosomes (FP2-exo) increased the expression of FGF2, confirming their wound-healing effect in vivo. Overall, the results of this study highlight the significant impact of FP2 on the proliferation of WJ MSCs and the anti-inflammatory and wound-healing effects of exosomes, suggesting potential applications beyond wound healing.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje