| Autor: |
Klimov DI; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1-3, Moscow 119991, Russia.; Enikolopov Institute of Synthetic Polymeric Materials, Russian Academy of Sciences, Profsoyuznaya st. 70, Moscow 117393, Russia., Zharikov AA; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1-3, Moscow 119991, Russia., Zezina EA; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1-3, Moscow 119991, Russia., Kotenkova EA; V.M. Gorbatov Federal Research Center for Food Systems, Talalikhina st., 26, Moscow 109316, Russia., Zaiko EV; V.M. Gorbatov Federal Research Center for Food Systems, Talalikhina st., 26, Moscow 109316, Russia., Bataeva DS; V.M. Gorbatov Federal Research Center for Food Systems, Talalikhina st., 26, Moscow 109316, Russia., Semenova AA; V.M. Gorbatov Federal Research Center for Food Systems, Talalikhina st., 26, Moscow 109316, Russia., Yushina YK; V.M. Gorbatov Federal Research Center for Food Systems, Talalikhina st., 26, Moscow 109316, Russia., Yaroslavov AA; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1-3, Moscow 119991, Russia., Zezin AA; Department of Chemistry, M.V. Lomonosov Moscow State University, Leninskie gory 1-3, Moscow 119991, Russia.; Enikolopov Institute of Synthetic Polymeric Materials, Russian Academy of Sciences, Profsoyuznaya st. 70, Moscow 117393, Russia. |
| Abstrakt: |
In order to control pathogenic microorganisms, three polymer compositions were prepared and tested. First, a water-soluble positively charged polycomplex was synthesized via the electrostatic binding of anionic polyacrylic acid to an excess of polyethylenimine to enhance the biocidal activity of the polycation. Second, an aqueous solution of AgNO 3 was added to the polycomplex, thus forming a ternary polycation-polyanion-Ag 1+ complex with an additional antimicrobial effect. Third, the resulting ternary complex was subjected to UV irradiation, which ensured the conversion of Ag 1+ ions into Ag nanoparticles ranging in size mainly from 10 to 20 nm. Aqueous solutions of the polymer compositions were added to suspensions of the Gram-positive bacteria S. aureus and the Gram-negative bacteria P. aeruginosa , with the following main results: (a) Upon the addition of the binary polycomplex, 30% or more of the cells survived after 20 h. (b) The ternary complex killed S. aureus bacteria but was ineffective against P. aeruginosa bacteria. (c) When the ternary complex with Ag nanoparticles was added, the percentage of surviving cells of both types did not exceed 0.03%. The obtained results are valuable for the development of antibacterial formulations. |
