| Autor: |
Rotondo A; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging (BIOMORF), University of Messina, Polo Universitario Annunziata, Viale Annunziata, 98168 Messina, Italy., Bartolomeo G; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging (BIOMORF), University of Messina, Polo Universitario Annunziata, Viale Annunziata, 98168 Messina, Italy., Spanò IM; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, V.le F. Stagno D'Alcontres 31, 98166 Messina, Italy., La Torre GL; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging (BIOMORF), University of Messina, Polo Universitario Annunziata, Viale Annunziata, 98168 Messina, Italy., Pellicane G; Department of Biomedical and Dental Sciences and Morpho-Functional Imaging (BIOMORF), University of Messina, Polo Universitario Annunziata, Viale Annunziata, 98168 Messina, Italy., Molinu MG; CNR-Istituto di Scienze delle Produzioni Alimentari (ISPA), Traversa La Crucca 3, Loc. Baldinca, Li Punti, 07040 Sassari, Italy., Culeddu N; CNR-Istituto di Chimica Biomolecolare (ICB), Traversa La Crucca 3, Loc. Baldinca, Li Punti, 07040 Sassari, Italy. |
| Abstrakt: |
Nuclear magnetic resonance (NMR) metabolomic analysis was applied to investigate the differences within nineteen Sicilian Nocellara del Belice monovarietal extra virgin olive oils (EVOOs), grown in two zones that are different in altitude and soil composition. Several classes of endogenous olive oil metabolites were quantified through a nuclear magnetic resonance (NMR) three-experiment protocol coupled with a yet-developed data-processing called MARA-NMR (Multiple Assignment Recovered Analysis by Nuclear Magnetic Resonance). This method, taking around one-hour of experimental time per sample, faces the possible quantification of different class of compounds at different concentration ranges, which would require at least three alternative traditional methods. NMR results were compared with the data of traditional analytical methods to quantify free fatty acidity (FFA), fatty acid methyl esters (FAMEs), and total phenol content. The presented NMR methodology is compared with traditional analytical practices, and its consistency is also tested through slightly different data treatment. Despite the rich literature about the NMR of EVOOs, the paper points out that there are still several advances potentially improving this general analysis and overcoming the other cumbersome and multi-device analytical strategies. Monovarietal EVOO's composition is mainly affected by pedoclimatic conditions, in turn relying upon the nutritional properties, quality, and authenticity. Data collection, analysis, and statistical processing are discussed, touching on the important issues related to the climate changes in Sicily and to the specific influence of pedoclimatic conditions. |
