Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells.

Autor: Eiras M; Lab. Fitovirologia e Fisiopatologia, Centro de Pesquisa de Sanidade Vegetal, Instituto Biológico, São Paulo, CEP 04014-002, SP, Brazil. marcelo.eiras@sp.gov.br.; Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), Valencia, 46022, Spain. marcelo.eiras@sp.gov.br., Aragonés V; Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), Valencia, 46022, Spain., Marqués J; Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), Valencia, 46022, Spain.; Azzur Group, Hatboro, USA., Gómez MD; Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), Valencia, 46022, Spain., Daròs JA; Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València), Valencia, 46022, Spain. jadaros@ibmcp.upv.es.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2024 Oct 15; Vol. 21 (1), pp. 254. Date of Electronic Publication: 2024 Oct 15.
DOI: 10.1186/s12985-024-02530-8
Abstrakt: Viroids that belong to genera Avsunviroid and Pelamovirod (family Avsunviroidae) replicate and accumulate in the chloroplasts of infected cells. In this report, we confirmed by RNA in situ hybridization using digoxigenin-UTP-labelled riboprobes that the positive strands of eggplant latent viroid (ELVd), the only member of genus Elaviroid within the family Avsunviroidae, also accumulate in the chloroplasts of infected cells. However, comparison of ELVd in situ hybridization signals with those from bona fide chloroplastic and nuclear non-coding RNAs, such as chloroplast 5S rRNA and U1 small nuclear RNA, supports the notion that this viroid is also present in the nuclei of infected cells. These results suggest that the subcellular localization of viroids within the family Avsunviroidae may be more complex than previously assumed with dynamic presence in several compartments during the infectious cycle.
(© 2024. The Author(s).)
Databáze: MEDLINE
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