Evaluation of an engineered vascular graft exhibiting somatic growth in lambs to model repair of absent pulmonary artery branch.

Autor: Syedain ZH; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA., Lahti M; Department of Surgery & Experimental Surgical Services, University of Minnesota, Minneapolis, MN, USA., Hiremath G; Department of Pediatrics & Masonic Children's Hospital, University of Minnesota, Minneapolis, MN, USA., Berry J; Department of Surgery & Experimental Surgical Services, University of Minnesota, Minneapolis, MN, USA., Carney JP; Department of Surgery & Experimental Surgical Services, University of Minnesota, Minneapolis, MN, USA., Schappa Faustich J; Department of Surgery & Experimental Surgical Services, University of Minnesota, Minneapolis, MN, USA.; Veterinary Clinical Sciences, University of Minnesota, Minneapolis, MN, USA., Shannon T; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA., Rivera A; Department of Biomedical Engineering, Florida International University, Miami, FL, USA., Wiputra H; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA., Shi Z; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA., Bianco R; Department of Surgery & Experimental Surgical Services, University of Minnesota, Minneapolis, MN, USA., MacIver R; Children's Hospitals and Clinics of Minnesota, Minneapolis, MN, USA., Mayer JE; Boston Children's Hospital and Department of Thoracic Surgery, Harvard Medical School, Boston, MA, USA., Tranquillo RT; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA. tranquillo@umn.edu.; Department of Chemical Engineering & Materials Science, University of Minnesota, Minneapolis, MN, USA. tranquillo@umn.edu.
Jazyk: angličtina
Zdroj: Communications medicine [Commun Med (Lond)] 2024 Oct 16; Vol. 4 (1), pp. 201. Date of Electronic Publication: 2024 Oct 16.
DOI: 10.1038/s43856-024-00614-8
Abstrakt: Background: Growth is the holy grail of tissue implants in pediatrics. No vascular graft currently in use for surgical repairs of congenital heart defects has somatic growth capacity.
Methods: Biologically-engineered grafts (6 mm) grown from donor ovine fibroblasts in a sacrificial fibrin gel were implanted into the left pulmonary branch of 3-month old lambs for 3, 6, and 18 months. A control group of Propaten® PTFE grafts was implanted for 6 months.
Results: The engineered grafts exhibit extensive site-appropriate recellularization after only 3 months and near-normal increase of diameter from the preimplant value of 6 mm to 12.9 mm and also a doubling of length from 6.0 mm to 13.0 mm at 6 months (n = 3) associated with apparent somatic graft growth (collagen content increase of 265% over 18-month, n = 2), along with excellent hemodynamics and no calcification, in contrast to the Propaten® grafts. The left-right flow distribution is nearly 50-50 for the engineered grafts at 6 months (n = 3) compared to about 20-80 for the Propaten® grafts (n = 3), which have less than one-half the diameter, a 6-fold higher pressure gradient, and stunted vascular development downstream of the graft. The engineered grafts exhibit a stable diameter over months 12-18 when the lambs become adult sheep (n = 2).
Conclusions: This study supports the use of these regenerative grafts with somatic growth capacity for clinical trial in patients born with a unilateral absent pulmonary artery branch, and it shows their potential for improving development of the downstream pulmonary vasculature.
(© 2024. The Author(s).)
Databáze: MEDLINE
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