The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3.

Autor: Han Z; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China., Wang R; State Key Laboratory of Stem Cell and Reproductive Biology, Key Laboratory of Organ Regeneration and Reconstruction, UCAS/IOZ/CAS, Beijing, China.; Beijing Institute of Stem Cell and Regenerative Medicine, Beijing, China.; Department of Reproductive Medicine, the First People's Hospital of Yunnan Province, Kunming, China., Chi P; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China., Zhang Z; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China., Min L; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China., Jiao H; Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong (Shenzhen), Shenzhen, China., Ou G; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China.; Clinical laboratory, West China Second University Hospital, Sichuan University, Chengdu, China., Zhou D; State Key Laboratory of Stem Cell and Reproductive Biology, Key Laboratory of Organ Regeneration and Reconstruction, UCAS/IOZ/CAS, Beijing, China.; Beijing Institute of Stem Cell and Regenerative Medicine, Beijing, China., Qin D; State Key Laboratory of Stem Cell and Reproductive Biology, Key Laboratory of Organ Regeneration and Reconstruction, UCAS/IOZ/CAS, Beijing, China.; Beijing Institute of Stem Cell and Regenerative Medicine, Beijing, China., Xu C; State Key Laboratory of Stem Cell and Reproductive Biology, Key Laboratory of Organ Regeneration and Reconstruction, UCAS/IOZ/CAS, Beijing, China.; Beijing Institute of Stem Cell and Regenerative Medicine, Beijing, China., Gao Z; Department of Obstetrics and Gynecology, Center for Reproductive Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Qi Q; Clinical laboratory, West China Second University Hospital, Sichuan University, Chengdu, China., Li J; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China., Lu Y; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China.; Department of Reproductive Medicine, West China Second University Hospital, Sichuan University, Chengdu, China., Wang X; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China.; NHC key Laboratory of Chronobiology, Sichuan University, Chengdu, China.; Development and Related Diseases of Women and Children Key Laboratory of Sichuan Province, Sichuan University, Chengdu, China., Chen J; Laboratory of Pediatric Surgery, Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, China., Yu X; State Key Laboratory of Stem Cell and Reproductive Biology, Key Laboratory of Organ Regeneration and Reconstruction, UCAS/IOZ/CAS, Beijing, China.; Beijing Institute of Stem Cell and Regenerative Medicine, Beijing, China., Hu H; Kobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong Kong (Shenzhen), Shenzhen, China., Li L; State Key Laboratory of Stem Cell and Reproductive Biology, Key Laboratory of Organ Regeneration and Reconstruction, UCAS/IOZ/CAS, Beijing, China. lil@ioz.ac.cn.; Beijing Institute of Stem Cell and Regenerative Medicine, Beijing, China. lil@ioz.ac.cn., Deng D; Key Laboratory of Birth Defects and Related Disease of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second University Hospital, Sichuan University, Chengdu, China. dengd@scu.edu.cn.; NHC key Laboratory of Chronobiology, Sichuan University, Chengdu, China. dengd@scu.edu.cn.; Development and Related Diseases of Women and Children Key Laboratory of Sichuan Province, Sichuan University, Chengdu, China. dengd@scu.edu.cn.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Oct 15; Vol. 15 (1), pp. 8887. Date of Electronic Publication: 2024 Oct 15.
DOI: 10.1038/s41467-024-53277-3
Abstrakt: The subcortical maternal complex (SCMC) is essential for safeguarding female fertility in mammals. Assembled in oocytes, the SCMC maintains the cleavage of early embryos, but the underlying mechanism remains unclear. Here, we report that 14-3-3, a multifunctional protein, is a component of the SCMC. By resolving the structure of the 14-3-3-containing SCMC, we discover that phosphorylation of TLE6 contributes to the recruitment of 14-3-3. Mechanistically, during maternal-to-embryo transition, the SCMC stabilizes 14-3-3 protein and contributes to the proper control of CDC25B, thus ensuring the activation of the maturation-promoting factor and mitotic entry in mouse zygotes. Notably, the SCMC establishes a conserved molecular link with 14-3-3 and CDC25B in human oocytes/embryos. This study discloses the molecular mechanism through which the SCMC regulates the cell cycle in early embryos and elucidates the function of the SCMC in mammalian early embryogenesis.
(© 2024. The Author(s).)
Databáze: MEDLINE
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