Avian deltacoronaviruses encode fusion-associated small transmembrane proteins that can induce syncytia formation.

Autor: Sartalamacchia K; Department of Pediatrics, Vanderbilt University Medical Center, 2200 Children's Way, Suite 2404, Nashville, TN, 37232, USA. Electronic address: k.sartalamacchia@vumc.org., Porter MS; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Medical Center North, 1161 21st Ave. South, Suite D-2220, Nashville, TN, 37232, USA., Veletanlic V; Department of Pediatrics, Vanderbilt University Medical Center, 2200 Children's Way, Suite 2404, Nashville, TN, 37232, USA., Ogden KM; Department of Pediatrics, Vanderbilt University Medical Center, 2200 Children's Way, Suite 2404, Nashville, TN, 37232, USA; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Medical Center North, 1161 21st Ave. South, Suite D-2220, Nashville, TN, 37232, USA. Electronic address: kristen.ogden@vumc.org.
Jazyk: angličtina
Zdroj: Virology [Virology] 2024 Dec; Vol. 600, pp. 110258. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1016/j.virol.2024.110258
Abstrakt: Fusion-associated small transmembrane (FAST) proteins are nonstructural viral proteins that induce cell-cell fusion. FAST proteins, which previously were identified in the genomes of double-stranded RNA viruses, typically contain an acylated N-terminal ectodomain, central transmembrane domain, and C-terminal endodomain with a polybasic region. Using sequence homology and protein motif prediction, we identified accessory proteins in a subset of avian deltacoronaviruses as putative FAST proteins. Transient expression of thrush coronavirus NS7b or common moorhen coronavirus NS7a, but not night heron coronavirus NS7b, induced cell-cell fusion. Syncytia were detected in primate kidney epithelial cells or fibroblasts but not chicken embryo fibroblasts, and addition of an N-terminal FLAG peptide to the proteins ablated fusion activity. These findings suggest that multiple avian deltacoronaviruses, positive-sense RNA viruses, encode nonstructural proteins that can mediate cell-cell fusion and share features with known FAST proteins. Additional studies are needed to understand contributions of these proteins to deltacoronavirus biology.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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