A new insight of blood vs. buccal DNA methylation in the forensic identification of monozygotic triplets.

Autor: El-Hossary NM; Biochemistry Division, Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt; Crime scene investigation (CSI) Laboratory, Ministry of Interior, Cairo 11517, Egypt., El-Desouky MA; Biochemistry Division, Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt. Electronic address: meldesouky@sci.cu.edu.eg., Sabry GM; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt., Omar MF; Crime scene investigation (CSI) Laboratory, Ministry of Interior, Cairo 11517, Egypt; Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt., Ali MY; Genomics program Department of Basic Research, Children's Cancer Hospital, Cairo 11562, Egypt; Clinical pharmacy Department, Faculty of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology, Cairo 12566, Egypt., Elzayat MG; Genomics program Department of Basic Research, Children's Cancer Hospital, Cairo 11562, Egypt., Hassan RE; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt., Mohamed RH; Biochemistry Department, Faculty of Science, Ain Shams University, Cairo 11566, Egypt., Rashidi FB; Biochemistry Division, Chemistry Department, Faculty of Science, Cairo University, Giza 12613, Egypt.
Jazyk: angličtina
Zdroj: Forensic science international [Forensic Sci Int] 2024 Nov; Vol. 364, pp. 112247. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1016/j.forsciint.2024.112247
Abstrakt: The case of the monozygotic (MZ) twin as a suspect demonstrates a practical problem in forensic casework. As the MZ twins are genetically identical, they share the same short tandem repeat (STR) profile. Many studies showed that older MZ twins have significant differences in overall content and genomic distribution of methylation between them. However, studies addressing the investigation of epigenetic MZ triplet differentiation in various forensic reference materials are lacking. Here, one triplet set of Egyptian MZ twins was used as an analog to a forensic case. The genome-wide methylation analysis was performed via the new Human Methylation EPIC BeadChip array. Following normalization methods, potential differentially methylated positions (DMPs) were discovered. This resulted in the detection of 24 potential DMPs in reference-type blood DNA and 11 potential DMPs in reference-type buccal DNA. Then, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses were performed to show the associated biological functions. Our findings revealed that the 35 potential DMPs were enriched in 283 significant GO terms. These terms are mainly enriched in the immune system. Overall, this study demonstrates the general feasibility of epigenetic MZ triplet differentiation in the forensic context and highlights that some potential DMPs identified in blood DNA were not informative in buccal DNA. This is due to various reasons, including the tissue specificity of DNA methylation.
Competing Interests: Declaration of Competing Interest All authors declare that there are no any competing financial interests in relation to this work.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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