TYPHON proteins are RAB-dependent mediators of the trans-Golgi network secretory pathway.

Autor:	Baral A; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden., Gendre D; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden., Aryal B; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden., Fougère L; Laboratoire de Biogénèse Membranaire, UMR5200, Université de Bordeaux, CNRS, Villenave d'Ornon 33140, France., Di Fino LM; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden., Ohori C; Natural Science Division, Faculty of Core Research, Ochanomizu University, Tokyo 112-8610, Japan., Sztojka B; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden., Uemura T; Natural Science Division, Faculty of Core Research, Ochanomizu University, Tokyo 112-8610, Japan., Ueda T; Division of Cellular Dynamics, National Institute for Basic Biology, Okazaki, Aichi 444-8585, Japan.; The Department of Basic Biology, SOKENDAI (The Graduate University for Advanced Studies), Okazaki, Aichi 444-8585, Japan., Marhavý P; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden., Boutté Y; Laboratoire de Biogénèse Membranaire, UMR5200, Université de Bordeaux, CNRS, Villenave d'Ornon 33140, France., Bhalerao RP; Department of Forest Genetics and Plant Physiology, Umeå Plant Science Centre, Swedish University of Agricultural Sciences, Umeå 901 87, Sweden.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Dec 23; Vol. 37 (1).
DOI:	10.1093/plcell/koae280
Abstrakt:	The trans-Golgi network (TGN), a key compartment in endomembrane trafficking, participates in both secretion to and endocytosis from the plasma membrane. Consequently, the TGN plays a key role in plant growth and development. Understanding how proteins are sorted for secretion or endocytic recycling at the TGN is critical for elucidating mechanisms of plant development. We previously showed that the protein ECHIDNA is essential for phytohormonal control of hypocotyl bending because it mediates secretion of cell wall components and the auxin influx carrier AUXIN RESISTANT 1 (AUX1) from the TGN. Despite the critical role of ECHIDNA in TGN-mediated trafficking, its mode of action remains unknown in Arabidopsis (Arabidopsis thaliana). We therefore performed a suppressor screen on the ech mutant. Here, we report the identification of TGN-localized TYPHON 1 (TPN1) and TPN2 proteins. A single amino acid change in either TPN protein causes dominant suppression of the ech mutant's defects in growth and AUX1 secretion, while also restoring wild-type (WT)-like ethylene-responsive hypocotyl bending. Importantly, genetic and cell biological evidence shows that TPN1 acts through RAS-ASSOCIATED BINDING H1b (RABH1b), a TGN-localized RAB-GTPase. These results provide insights into ECHIDNA-mediated secretory trafficking of cell wall and auxin carriers at the TGN, as well as its role in controlling plant growth. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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