Intermittent Fasting Ameliorates Age-Induced Morphological Changes in Aged Albino Rat Kidney via Autophagy Activation and Reduction of Apoptosis and Inflammation.

Autor:	Rifaai RA; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Cairo Aswan Agricultural Rd, Minia, 61519, Egypt., El-Tahawy NFG; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Cairo Aswan Agricultural Rd, Minia, 61519, Egypt., Abozaid SMM; Department of Human Anatomy and Embryology, Faculty of Medicine, Minia University, Cairo Aswan Agricultural Rd, 61519, Egypt., Abdelwahab A; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Cairo Aswan Agricultural Rd, Minia, 61519, Egypt.
Jazyk:	angličtina
Zdroj:	Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2024 Oct 15. Date of Electronic Publication: 2024 Oct 15.
DOI:	10.1093/mam/ozae102
Abstrakt:	Aging is a biological process with gradual decrease of cell function. Kidneys are one of the organs with higher susceptibility to the development of age-dependent tissue damage. Intermittent fasting has several beneficial effects on age-related degenerative changes. The aim of this study was to investigate the possible beneficial effect of intermittent fasting in delaying age-related renal changes and the possible mechanisms of this effect. Thirty male albino rats were classified into three groups: control, adult rats aged 3 months; aged group, 15-month-old rats and maintained until the age of 18 months; and intermittent fasting-aged groups, 15-month-old rats maintained on intermittent fasting for 3 months. Kidneys were processed for histological and immunohistochemical study. Aging resulted in a significant reduction in renal function and significant several degenerative changes in renal corpuscles and tubules which showed abnormal histological structure with increased collagen deposition. Aging caused significant reduction in the expression of autophagic marker light chain 3 with increased expression of active caspase-3 and inducible nitric oxide synthase. Intermittent fasting significantly improved these age-related renal changes. Intermittent fasting effectively prevents age-related renal changes through the reduction of age-related oxidative stress, inflammation, apoptosis, and activation of autophagy. Competing Interests: Conflict of Interest The authors declare that they have no competing interest. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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