| Autor: |
Jeong Y; Department of Molecular and Human Genetics and., Deveza L; Department of Orthopedic Surgery, Baylor College of Medicine, Houston, Texas, USA., Ortinau L; Department of Molecular and Human Genetics and., Lei K; Department of Molecular and Human Genetics and., Dawson JR; Department of Orthopedic Surgery, Baylor College of Medicine, Houston, Texas, USA., Park D; Department of Molecular and Human Genetics and. |
| Jazyk: |
angličtina |
| Zdroj: |
JCI insight [JCI Insight] 2024 Nov 22; Vol. 9 (22). Date of Electronic Publication: 2024 Nov 22. |
| DOI: |
10.1172/jci.insight.173831 |
| Abstrakt: |
Human periosteal skeletal stem cells (P-SSCs) are critical for cortical bone maintenance and repair. However, their in vivo identity, molecular characteristics, and specific markers remain unknown. Here, single-cell sequencing revealed human periosteum contains SSC clusters expressing known SSC markers, podoplanin (PDPN) and PDGFRA. Notably, human P-SSCs, but not bone marrow SSCs, selectively expressed identified markers low density lipoprotein receptor-related protein 1 (LRP1) and CD13. These LRP1+CD13+ human P-SSCs were perivascular cells with high osteochondrogenic but minimal adipogenic potential. Upon transplantation into bone injuries in mice, they preserved self-renewal capability in vivo. Single-cell analysis of mouse periosteum further supported the preferential expression of LRP1 and CD13 in Prx1+ P-SSCs. When Lrp1 was conditionally deleted in Prx1 lineage cells, it led to severe bone deformity, short stature, and periosteal defects. By contrast, local treatment with an LRP1 agonist at the injury sites induced early P-SSC proliferation and bone healing. Thus, human and mouse periosteum contains unique osteochondrogenic stem cell subsets, and these P-SSCs express specific markers, LRP1 and CD13, with a regulatory mechanism through LRP1 that enhances P-SSC function and bone repair. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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