AUXIN RESPONSE FACTOR 2 mediates repression of strawberry receptacle ripening via auxin-ABA interplay.

Autor:	Li BJ; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning, 530004, China.; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China., Shi YN; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China., Xiao YN; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China., Jia HR; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China., Yang XF; Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310058, China., Dai ZR; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China., Sun YF; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China., Shou JH; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China., Jiang GH; Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310058, China., Grierson D; State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.; Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK., Chen KS; College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; Zhejiang Key Laboratory of Horticultural Crop Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.; State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Oct 15. Date of Electronic Publication: 2024 Oct 15.
DOI:	10.1093/plphys/kiae510
Abstrakt:	Cultivated strawberry (Fragaria × ananassa) is a popular, economically important fruit. The ripening of the receptacle (pseudocarp), the main edible part, depends on endogenously produced abscisic acid (ABA) and is suppressed by the high level of auxin produced from achenes (true fruit) during early development. However, the mechanism whereby auxin regulates receptacle ripening through inhibiting ABA biosynthesis remains unclear. Here, we identified AUXIN RESPONSE FACTOR 2 (FaARF2), which showed decreased expression with reduced auxin content in the receptacle, leading to increased ABA levels and accelerated ripening. Dual-luciferase, yeast one-hybrid, and electrophoretic mobility shift assays demonstrated that FaARF2 could bind to the AuxRE element in the promoter of 9-CIS-EPOXYCAROT-ENOID DIOXYGENASE 1 (FaNCED1), a key ABA biosynthetic gene, to suppress its transcriptional activity. Transiently overexpressing FaARF2 in the receptacles decreased FaNCED1 expression and ABA levels, resulting in inhibition of receptacle ripening and of development of quality attributes, such as pigmentation, aroma, and sweetness. This inhibition caused by overexpressing FaARF2 was partially recovered by the injection of exogenous ABA; conversely, transient silencing of FaARF2 using RNA interference produced the opposite results. The negative targeting of FaNCED1 by FaARF2 is a key link between auxin-ABA interactions and regulation of strawberry ripening. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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