Fabrication of endothelialized capillary-like microchannel networks using sacrificial thermoresponsive microfibers.

Autor:	Rector Iv JA; Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, United States of America., McBride L; Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, United States of America., Weber CM; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, United States of America., Grossman K; Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, United States of America., Sorets A; Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States of America., Ventura-Antunes L; School of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States of America., Holtz I; Department of Cognitive Studies, Vanderbilt University, Nashville, TN, United States of America.; Department of Medicine, Health, and Society, Vanderbilt University, Nashville, TN, United States of America., Young K; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, United States of America., Schrag M; School of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States of America., Lippmann ES; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, United States of America.; Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, United States of America., Bellan LM; Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, United States of America.; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, United States of America.
Jazyk:	angličtina
Zdroj:	Biofabrication [Biofabrication] 2024 Nov 19; Vol. 17 (1). Date of Electronic Publication: 2024 Nov 19.
DOI:	10.1088/1758-5090/ad867d
Abstrakt:	In the body, capillary beds fulfill the metabolic needs of cells by acting as the sites of diffusive transport for vital gasses and nutrients. In artificial tissues, replicating the scale and complexity of capillaries has proved challenging, especially in a three-dimensional context. In order to better develop thick artificial tissues, it will be necessary to recreate both the form and function of capillaries. Here we demonstrate a top-down method of patterning hydrogels using sacrificial templates formed from thermoresponsive microfibers whose size and architecture approach those of natural capillaries. Within the resulting microchannels, we cultured endothelial monolayers that remain viable for over three weeks and exhibited functional barrier properties. Additionally, we cultured endothelialized microchannels within hydrogels containing fibroblasts and characterized the viability of the co-cultures to demonstrate this approach's potential when applied to cell-laden hydrogels. This method represents a step forward in the evolution of artificial tissues and a path towards producing viable capillary-scale microvasculature for engineered organs. (Creative Commons Attribution license.)
Databáze:	MEDLINE
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