Influence of Storage Methods on the Vitality and Growth Rate of Macrofungi.

Autor: Komissarov NS; Moscow State University, Moscow, Russia. macoloams@gmail.com., Dyakov MY; Moscow State University, Moscow, Russia. max_fungi@mail.ru., Garibova LV; Moscow State University, Moscow, Russia. gariblv@yandex.ru.
Jazyk: angličtina
Zdroj: Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections [Dokl Biol Sci] 2024 Oct 11. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1134/S0012496624701278
Abstrakt: The work contains a comparative analysis of methods for storing pure cultures of macrofungi. The study used 20 species of macrofungi from various taxonomic and ecological-trophic groups. Storage was carried out using five methods: serial subculturing, storage under a layer of distilled water and three cryopreservation protocols: a protocol using blocks of agar medium, a "perlite protocol," and a "grain protocol." For the selected cryostorage methods, various cryoprotective compounds (glycerol, trehalose) were used. Radial growth rate was used as a criterion for the state of crops. The values of the radial growth rate obtained immediately after isolation of the pure culture were chosen as the control. It has been shown that the most favorable for preserving the physiological activity of cultures are the storage method under a layer of distilled water, "perlite" and "grain" cryopreservation protocols.
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Databáze: MEDLINE
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