Identification and Characterization of Innate Immunity in Actinidia melanandra in Response to Pseudomonas syringae pv. actinidiae.

Autor: Hemara LM; School of Biological Sciences, The University of Auckland, Auckland, New Zealand.; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Chatterjee A; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Yeh SM; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Chen RKY; The New Zealand Institute for Plant and Food Research Limited, Palmerston North, New Zealand., Hilario E; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Lievre LL; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand.; Department of Biochemistry, University of Otago, Dunedin, New Zealand., Crowhurst RN; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Bohne D; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Arshed S; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Patterson HR; School of Biological Sciences, The University of Auckland, Auckland, New Zealand.; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Barrett-Manako K; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Thomson S; The New Zealand Institute for Plant and Food Research Limited, Lincoln Research Centre, New Zealand., Allan AC; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Brendolise C; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Chagné D; The New Zealand Institute for Plant and Food Research Limited, Palmerston North, New Zealand., Templeton MD; School of Biological Sciences, The University of Auckland, Auckland, New Zealand.; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Tahir J; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand., Jayaraman J; The New Zealand Institute for Plant and Food Research Limited, Mount Albert Research Centre, New Zealand.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Oct 13. Date of Electronic Publication: 2024 Oct 13.
DOI: 10.1111/pce.15189
Abstrakt: Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) has decimated kiwifruit orchards growing susceptible kiwifruit Actinidia chinensis varieties. Effector loss has occurred recently in Psa3 isolates from resistant kiwifruit germplasm, resulting in strains capable of partially overcoming resistance present in kiwiberry vines (Actinidia arguta, Actinidia polygama, and Actinidia melanandra). Diploid male A. melanandra recognises several effectors, sharing recognition of at least one avirulence effector (HopAW1a) with previously studied tetraploid kiwiberry vines. Sequencing and assembly of the A. melanandra genome enabled the characterisation of the transcriptomic response of this non-host to wild-type and genetic mutants of Psa3. A. melanandra appears to mount a classic effector-triggered immunity (ETI) response to wildtype Psa3 V-13, as expected. Surprisingly, the type III secretion (T3SS) system-lacking Psa3 V-13 ∆hrcC strain did not appear to trigger pattern-triggered immunity (PTI) despite lacking the ability to deliver immunity-suppressing effectors. Contrasting the A. melanandra responses to an effectorless Psa3 V-13 ∆33E strain and to Psa3 V-13 ∆hrcC suggested that PTI triggered by Psa3 V-13 was based on the recognition of the T3SS itself. The characterisation of both ETI and PTI branches of innate immunity responses within A. melanandra further enables breeding for durable resistance in future kiwifruit cultivars.
(© 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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