Sorghum bicolor Extracellular Vesicle Isolation, Labeling, and Correlative Light and Electron Microscopy.
| Autor: | Adekanye D; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA., Chaya T; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA., Caplan JL; Department of Plant and Soil Sciences, University of Delaware, Newark, DE, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Bio-protocol [Bio Protoc] 2024 Oct 05; Vol. 14 (19), pp. e5083. Date of Electronic Publication: 2024 Oct 05 (Print Publication: 2024). |
| DOI: | 10.21769/BioProtoc.5083 |
| Abstrakt: | Extracellular vesicles are membrane-bound organelles that play crucial roles in intercellular communication and elicit responses in the recipient cell, such as defense responses against pathogens. In this study, we have optimized a protocol for isolating extracellular vesicles (EVs) from Sorghum bicolor apoplastic wash. We characterized the EVs using fluorescence microscopy and correlative light and electron microscopy. Key features • Allows the isolation of extracellular vesicles from the monocot plant Sorghum bicolor . • Labels isolated extracellular vesicles with fluorescent dyes for easy characterization with light microscopy. • Validates dye labeling and further characterizes extracellular vesicles using a correlative light and electron microscopy approach. Competing Interests: Competing interestsThere are no conflicts of interest or competing interest. (©Copyright : © 2024 The Authors; This is an open access article under the CC BY-NC license.) |
| Databáze: | MEDLINE |
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