Differential type I and type III interferon expression profiles in rheumatoid and juvenile idiopathic arthritis.

Autor:	Malik AE; IWK Health Centre, Halifax, NS, Canada.; Department of Pediatrics, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada., Slauenwhite D; IWK Health Centre, Halifax, NS, Canada.; Department of Pediatrics, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada., McAlpine SM; IWK Health Centre, Halifax, NS, Canada.; Department of Pediatrics, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada., Hanly JG; Division of Rheumatology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada.; Queen Elizabeth II Health Sciences Centre, Halifax, NS, Canada., Marshall JS; Department of Microbiology & Immunology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada., Dérfalvi B; IWK Health Centre, Halifax, NS, Canada.; Department of Pediatrics, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada., Issekutz TB; IWK Health Centre, Halifax, NS, Canada.; Department of Pediatrics, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada.
Jazyk:	angličtina
Zdroj:	Frontiers in medicine [Front Med (Lausanne)] 2024 Sep 27; Vol. 11, pp. 1466397. Date of Electronic Publication: 2024 Sep 27 (Print Publication: 2024).
DOI:	10.3389/fmed.2024.1466397
Abstrakt:	Background: The role of type I and type III interferons (IFNs) in rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) is still poorly understood. The objective of this study was to examine the hypothesis that IFN expression profiles in the peripheral blood differ between subsets of arthritic subjects. Multiple type I and type III IFNs were examined in patients with RA and JIA, as well as among subtypes of JIA. Methods: Treatment-naïve RA and JIA patients were enrolled. Droplet digital PCR was used to measure the expression of type I, II, and III interferons in blood and synovial fluid leukocytes. Dendritic cell subsets were isolated from synovial fluid to examine IFN expression in each subset. Additionally, synovial mononuclear cells and JIA-derived fibroblast-like synoviocytes were stimulated with TNF, IFNγ, and poly(I:C) to examine inducible IFN expression. Results: The predominant type I IFN gene expressed by blood leukocytes was IFNκ and was significantly lower in RA than JIA and controls. Oligoarticular and psoriatic JIA subgroups showed higher IFNκ expression compared to polyarticular JIA and RA. JIA synovial fluid leukocytes expressed abundant IFNγ and type III IFNs ( IFNλ1, IFNλ3 ), with distinct dendritic cell subset contributions. JIA fibroblast-like synoviocytes produced IFNβ, IFNλ1, and IFNλ2 mRNA upon poly(I:C) stimulation. Conclusion: This study revealed differences in IFN expression patterns in RA and JIA, with notable differences between JIA subtypes. The expression levels of IFNκ, IFNγ , IFNλ1 and IFNλ3 in JIA suggest specific roles in disease pathology, influenced by disease subtype and joint microenvironment. This study contributes to understanding IFN-mediated mechanisms in arthritis, potentially guiding targeted therapeutic strategies. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Malik, Slauenwhite, McAlpine, Hanly, Marshall, Dérfalvi and Issekutz.)
Databáze:	MEDLINE
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