| Autor: |
Maas LMI; Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, 04103 Leipzig, Germany., Winter M; Rio Negro Research and Transfer Center (CONICET-UNRN), National University of Rio Negro, 8500 Viedma, Argentina., Herrmann V; Veterinary clinic 'La Victoria', 8536 Valcheta, Argentina., Abate SD; Rio Negro Research and Transfer Center (CONICET-UNRN), National University of Rio Negro, 8500 Viedma, Argentina., Obiegala A; Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, 04103 Leipzig, Germany., Nava S; Dairy Chain Research Institute (IdICaL; CONICET-INTA), 2300 Rafaela, Argentina., Sebastian PS; Dairy Chain Research Institute (IdICaL; CONICET-INTA), 2300 Rafaela, Argentina. |
| Abstrakt: |
The aim of this study was to examine the presence of tick-borne rickettsial bacteria in Rhipicephalus sanguineus sensu stricto ticks collected from dogs in the Patagonian region of Argentina. Fourteen stray dogs from Valcheta, Río Negro province, Argentina were examined for the presence of R. sanguineus s.s. ticks. Ninety ticks were collected and identified to species level. DNA was extracted and analysed by conventional PCR assays for the presence of tick-borne bacteria belonging to the genera Anaplasma , Ehrlichia and Rickettsia . Thirty-three tick pools were tested by different PCR assays of which 3 were positive for Anaplasmataceae bacteria. From the 3 Anaplasmataceae positive samples, 2 partial 16S rDNA sequences were generated and belonging to Anaplasma platys , the causative agent of canine cyclic thrombocytopenia. Two tick samples were positive in Rickettsia specific PCR assays and were identified by phylogenetic analysis as Rickettsia massiliae , a member of the spotted fever group rickettsiae. The results of this study demonstrate the molecular detection of 2 rickettsial bacteria in R. sanguineus s.s. in a region of Argentina where no data were available so far. |
