Recurrent tick bites induce high IgG1 antibody responses to α-Gal in sensitized and non-sensitized forestry employees in Luxembourg.

Autor: Chakrapani N; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg., Swiontek K; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg., Hübschen JM; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg., Fischer J; Faculty of Medicine, Department of Dermatology, Eberhard Karls University of Tübingen, Tübingen, Germany., Ruiz-Castell M; Department of Precision Health, Luxembourg Institute of Health, Strassen, Luxembourg., Codreanu-Morel F; The Immunology-Allergology Unit, Center Hospitalier Luxembourg, Luxembourg, Luxembourg., Hannachi F; The Immunology-Allergology Unit, Center Hospitalier Luxembourg, Luxembourg, Luxembourg., Morisset M; The Immunology-Allergology Unit, Center Hospitalier Luxembourg, Luxembourg, Luxembourg., Ollert M; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.; Department of Dermatology and Allergy Center, Odense Research Center for Anaphylaxis, University of Southern Denmark, Odense, Denmark., Kuehn A; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg., Muller CP; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg., Hilger C; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg.
Jazyk: angličtina
Zdroj: Clinical and translational allergy [Clin Transl Allergy] 2024 Oct; Vol. 14 (10), pp. e12396.
DOI: 10.1002/clt2.12396
Abstrakt: Background: The α-Gal syndrome (AGS) is characterized by the presence of specific IgE-antibodies to the carbohydrate galactose-α-1,3-galactose (α-Gal). Sensitization to α-Gal has been associated with tick bites and individuals exposed to ticks have an elevated risk of sensitization. The aim of this study was to analyze IgG and IgE antibody responses to α-Gal in a high-risk cohort of forestry employees (FE) in Luxembourg.
Methods: Questionnaires and serum samples of FE from Luxembourg (n = 219) were retrospectively analyzed. α-Gal specific IgE was quantified by ImmunoCAP, α-Gal specific IgG and subclasses IgG 1-4 were determined by ELISA. Additionally, sera from population-based controls (n = 150) and two groups of food-allergic patients, patients with AGS (n = 45) and fish-allergic patients (n = 22) were assessed for IgG antibody responses to α-Gal and cod extract.
Results: Twenty-one percent of FE was sensitized to α-Gal (sIgE ≥ 0.1 kU A /L). Both sensitized and non-sensitized FE exhibited high levels of α-Gal specific IgG, IgG1 and IgG3 compared with controls, indicating a stimulation of IgG responses by recurrent tick bites, independent of the sensitization status. AGS patients had the highest levels of IgG1 and IgG2 antibodies, whereas the profile of fish-allergic patients was similar to the profile of the controls for which anti-α-Gal responses were dominated by IgG2 antibodies. α-Gal sIgG4 levels were either very low or undetectable in all groups.
Conclusion: Our study provides evidence for a continuous stimulation of α-Gal related immune responses by repeated tick bites, translating into highly elevated levels of IgG1 antibodies directed against α-Gal.
(© 2024 The Author(s). Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje