Aerosol immunization with influenza matrix, nucleoprotein, or both prevents lung disease in pig.

Autor: Vatzia E; The Pirbright Institute, Pirbright, UK., Paudyal B; The Pirbright Institute, Pirbright, UK., Dema B; Nuffield Department of Medicine, University of Oxford, Oxford, UK., Carr BV; The Pirbright Institute, Pirbright, UK., Sedaghat-Rostami E; The Pirbright Institute, Pirbright, UK., Gubbins S; The Pirbright Institute, Pirbright, UK., Sharma B; The Pirbright Institute, Pirbright, UK., Moorhouse E; The Pirbright Institute, Pirbright, UK., Morris S; Nuffield Department of Medicine, University of Oxford, Oxford, UK., Ulaszewska M; Nuffield Department of Medicine, University of Oxford, Oxford, UK., MacLoughlin R; Aerogen Ltd., Galway, Ireland., Salguero FJ; United Kingdom Health Security Agency, UKHSA-Porton Down, Salisbury, UK., Gilbert SC; Nuffield Department of Medicine, University of Oxford, Oxford, UK., Tchilian E; The Pirbright Institute, Pirbright, UK. elma.tchilian@pirbright.ac.uk.
Jazyk: angličtina
Zdroj: NPJ vaccines [NPJ Vaccines] 2024 Oct 13; Vol. 9 (1), pp. 188. Date of Electronic Publication: 2024 Oct 13.
DOI: 10.1038/s41541-024-00989-8
Abstrakt: Current influenza vaccines are strain-specific and require frequent updates to combat new strains, making a broadly protective influenza vaccine (BPIV) highly desirable. A promising strategy is to induce T-cell responses against internal proteins conserved across influenza strains. In this study, pH1N1 pre-exposed pigs were immunized by aerosol using viral vectored vaccines (ChAdOx2 and MVA) expressing matrix (M1) and nucleoprotein (NP). Following H3N2 challenge, all immunizations (M1, NP or NPM1) reduced lung pathology, but M1 alone offered the greatest protection. NP or NPM1 immunization induced both T-cell and antibody responses. M1 immunization generated no detectable antibodies but elicited M1-specific T-cell responses, suggesting T cell-mediated protection. Additionally, a single aerosol immunization with the ChAdOx vaccine encoding M1, NP and neuraminidase reduced lung pathology. These findings provide insights into BPIV development using a relevant large natural host, the pig.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: