Evaluation of Minimum-to-Severe Global and Macrovesicular Steatosis in Human Liver Specimens: A Portable Ambient Light-Compatible Spectroscopic Probe.

Autor: Guo H; Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Canada.; Department of Medical Physics, Nova Scotia Health Authority, Halifax, Canada.; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA., Stueck AE; Department of Pathology, Dalhousie University, Halifax, Canada., Doppenberg JB; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., Chae YS; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.; Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands., Tikhomirov AB; Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Canada., Zeng H; Imaging Unit - Integrative Oncology Department, BC Cancer Research Center, Vancouver, Canada., Engelse MA; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands.; Department of Internal Medicine, Leiden University Medical Center, Leiden, The Netherlands., Gala-Lopez BL; Department of Surgery, Dalhousie University, Halifax, Canada., Mahadevan-Jansen A; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA.; Vanderbilt Biophotonics Center, Nashville, Tennessee, USA., Alwayn IPJ; Transplant Center, Leiden University Medical Center, Leiden, The Netherlands., Locke AK; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA.; Vanderbilt Biophotonics Center, Nashville, Tennessee, USA.; Department of Chemistry, Vanderbilt University, Nashville, Tennessee, USA., Hewitt KC; Department of Physics and Atmospheric Science, Dalhousie University, Halifax, Canada.
Jazyk: angličtina
Zdroj: Journal of biophotonics [J Biophotonics] 2024 Oct 13, pp. e202400292. Date of Electronic Publication: 2024 Oct 13.
DOI: 10.1002/jbio.202400292
Abstrakt: Background and Aims: Hepatic steatosis (HS), particularly macrovesicular steatosis (MaS), influences transplant outcomes. Accurate assessment of MaS is crucial for graft selection. While traditional assessment methods have limitations, non-invasive spectroscopic techniques like Raman and reflectance spectroscopy offer promise. This study aimed to evaluate the efficacy of a portable ambient light-compatible spectroscopic system in assessing global HS and MaS in human liver specimens.
Methods: A two-stage approach was employed on thawed snap-frozen human liver specimens under ambient room light: biochemical validation involving a comparison of fat content from Raman and reflectance intensities with triglyceride (TG) quantifications and histopathological validation, contrasting Raman-derived fat content with evaluations by an expert pathologist and a "Positive Pixel Count" algorithm. Raman and reflectance intensities were combined to discern significant (≥ 10%) discrepancies in global HS and MaS.
Results: The initial set of 16 specimens showed a positive correlation between Raman and reflectance-derived fat content and TG quantifications. The Raman system effectively differentiated minimum-to-severe global and macrovesicular steatosis in the subsequent 66 specimens. A dual-variable prediction algorithm was developed, effectively classifying significant discrepancies (> 10%) between algorithm-estimated global HS and pathologist-estimated MaS.
Conclusion: Our study established the viability and reliability of a portable spectroscopic system for non-invasive HS and MaS assessment in human liver specimens. The compatibility with ambient light conditions and the ability to address limitations of previous methods marks a significant advancement in this field. By offering promising differentiation between global HS and MaS, our system introduces an innovative approach to real-time and quantitative donor HS assessments. The proposed method holds the promise of refining donor liver assessment during liver recovery and ultimately enhancing transplantation outcomes.
(© 2024 The Author(s). Journal of Biophotonics published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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