Assessing nanotoxicity of food-relevant particles: A comparative analysis of cellular responses in cell monolayers versus 3D gut epithelial cultures.

Autor: Gautam A; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore., Lim HK; Innovations in Food and Chemical Safety (IFCS) Programme, Agency for Science, Technology and Research, Singapore; Skin Research Institute of Singapore (SRIS), Agency for Science, Technology and Research, Singapore; Future Ready Food Safety Hub (a Joint Initiative of A∗STAR, SFA & NTU), Nanyang Technological University, Singapore., Li JJ; National Centre for Food Science, Singapore Food Agency, Singapore., Hughes CO; Innovations in Food and Chemical Safety (IFCS) Programme, Agency for Science, Technology and Research, Singapore; Skin Research Institute of Singapore (SRIS), Agency for Science, Technology and Research, Singapore; Future Ready Food Safety Hub (a Joint Initiative of A∗STAR, SFA & NTU), Nanyang Technological University, Singapore., Yeo CWS; National Centre for Food Science, Singapore Food Agency, Singapore., Rakshit M; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore., Leavesley DI; Skin Research Institute of Singapore (SRIS), Agency for Science, Technology and Research, Singapore., Lim MJS; Future Ready Food Safety Hub (a Joint Initiative of A∗STAR, SFA & NTU), Nanyang Technological University, Singapore; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research, Singapore., Tan JCW; Future Ready Food Safety Hub (a Joint Initiative of A∗STAR, SFA & NTU), Nanyang Technological University, Singapore., Tan LY; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore., Chan JSH; National Centre for Food Science, Singapore Food Agency, Singapore., Smith BPC; Innovations in Food and Chemical Safety (IFCS) Programme, Agency for Science, Technology and Research, Singapore; Future Ready Food Safety Hub (a Joint Initiative of A∗STAR, SFA & NTU), Nanyang Technological University, Singapore; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research, Singapore; Monell Chemical Senses Center, 3500 Market Street, Pennsylvania, 19104, USA. Electronic address: bsmith@monell.org., Ng KW; School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore; Skin Research Institute of Singapore (SRIS), Agency for Science, Technology and Research, Singapore; Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, CleanTech One, Singapore. Electronic address: kwng@ntu.edu.sg.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2024 Oct 11; Vol. 193, pp. 115055. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1016/j.fct.2024.115055
Abstrakt: Engineered nanoparticles (NPs) are extensively used in the food industry, yet safety concerns remain. The lack of validated methodologies is a bottleneck towards resolving this uncertainty. Hence, the current study aims to compare two cell models by examining the toxicological impacts of two food-relevant NPs (SiO 2 and Ag) on intestinal epithelia using monolayer Caco-2 cells and full-thickness 3D tissue models of human small intestines (EpiIntestinal™). Comprehensive characterization and dosimetric analysis of the NPs were performed to determine effective doses and model realistic exposures. Neither genotoxicity nor cytotoxicity were detected in the 3D tissues after NP treatment, while the 2D cultures exhibited cytotoxic response from Ag NP treatment for 24 h at 1 μg/ml. Hyperspectral imaging and transmission electron microscopy confirmed uptake of both NPs by cells in both 2D and 3D culture models. Ag NPs caused an increase in autophagy, whereas SiO 2 NPs induced increased cytoplasmic vacuolization. Based on realistic exposure levels studied, the 3D small intestinal tissue model was found to be more resilient to NP treatment compared to 2D cell monolayers. This comparative approach towards toxicological assessment of food relevant NPs could be used as a framework for future analysis of NP behavior and nanotoxicity in the gut.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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