Dynamic alterations in T-lymphocyte subsets assessed by flow cytometry in chickens following exposure to infectious bursal disease virus: A systematic review.

Autor: Pedrazzoli S; Department of Veterinary Medical Sciences, University of Bologna, 40064, Ozzano dell'Emilia BO, Italy. Electronic address: sara.pedrazzoli2@unibo.it., Graziosi G; Department of Veterinary Medical Sciences, University of Bologna, 40064, Ozzano dell'Emilia BO, Italy. Electronic address: giulia.graziosi2@unibo.it., Salaroli R; Department of Veterinary Medical Sciences, University of Bologna, 40064, Ozzano dell'Emilia BO, Italy. Electronic address: roberta.salaroli@unibo.it., Catelli E; Department of Veterinary Medical Sciences, University of Bologna, 40064, Ozzano dell'Emilia BO, Italy. Electronic address: elena.catelli@unibo.it., Lupini C; Department of Veterinary Medical Sciences, University of Bologna, 40064, Ozzano dell'Emilia BO, Italy. Electronic address: caterina.lupini@unibo.it.
Jazyk: angličtina
Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2024 Oct 11; Vol. 162, pp. 105280. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1016/j.dci.2024.105280
Abstrakt: Infectious bursal disease virus (IBDV) is a significant pathogen in poultry, causing acute immunosuppressive disease in young chickens. While B-lymphocyte involvement in IBDV pathogenesis is known, the role of T-cells is incompletely understood. This systematic review presents the alterations in chicken T-lymphocyte subsets after IBDV exposure, assessed by flow cytometry analysis. Four databases were queried for identifying eligible studies focused on experimental infections measuring T-lymphocyte changes in the bursa of Fabricius, spleen, thymus, and peripheral blood mononuclear cells. Of 488 studies found, 25 met the pre-established criteria and were included in the qualitative synthesis of results. Most studies analysed T-lymphocyte responses during the acute phase of IBDV infection, primarily focusing on CD4 + and CD8 + T-cells. Other subsets, such as γδ T-cells and double-positive CD4 + CD8 + T-cells, were less frequently investigated. An increase in T-lymphocytes was noted in the bursa of Fabricius, suggesting their active role in viral clearance. In the spleen, CD4 + T-cells commonly increased, while CD8 + responses varied among studies. Increased levels in T-cells were also noted during the chronic infection in the bursa of Fabricius, possibly due to persistent viral antigens. Overall, variations in flow cytometry methods and T-cell output reporting were noted among studies. Based on the data collected, further investigation into diverse T-cell subpopulations beyond CD4 + and CD8 + is needed, as well as the standardization of flow cytometry assays in chickens.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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