Development of a label-free electrochemical aptasensor for Rift Valley fever virus detection.

Autor: Alharbi MA; Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia., Rhouati A; Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia.; Bioengineering Laboratory, Higher National School of Biotechnology, Constantine, Algeria., Zourob M; Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Road, Riyadh, 11533, Saudi Arabia. mzourob@alfaisal.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 12; Vol. 14 (1), pp. 23892. Date of Electronic Publication: 2024 Oct 12.
DOI: 10.1038/s41598-024-74314-7
Abstrakt: In this research, we describe the first aptasensor for the detection of the Rift Valley Fever virus (RVFV). The process involved the selection of aptamers through the systematic evolution of ligands by the exponential enrichment (SELEX) technique. After 12 rounds of selection, 6 aptamers were selected and the corresponding binding affinities were assessed using fluorescence binding assays, revealing dissociation constants ranging from 15.45 to 40.98 nM. Notably, among the aptamers, RV2 and RV3 exhibited the highest binding affinities toward RVFV, with dissociation constants of 15.45 and 18.62 nM, respectively. Thiol-modified aptamers were subsequently immobilized onto screen-printed gold electrodes, facilitating the label-free detection of RVFV through square wave voltammetry. The voltammetric aptasensor demonstrated an excellent sensitivity, with a detection limit of 0.015 ng/mL. In addition, cross-reactivity assessments were conducted, where negligible response was obtained when the aptasensor was exposed to non-specific proteins.
(© 2024. The Author(s).)
Databáze: MEDLINE
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