Bioactivity of Talisia esculenta extracts: Antioxidant and anti-inflammatory action on RAW 264.7 macrophages and protective potential on the zebrafish exposed to oxidative stress inducers.

Autor: Cordeiro MLDS; Laboratório de Transformação de Plantas e Análise Em Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil., Queiroz Aquino-Martins VG; Laboratório de Transformação de Plantas e Análise Em Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil., Silva APD; Laboratório de Transformação de Plantas e Análise Em Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil., Paiva WS; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil; Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil., Silva MMCL; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil; Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil., Luchiari AC; FishLab, Departamento de Fisiologia e Comportamento, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, 59078-970, Brazil., Rocha HAO; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil; Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil., Scortecci KC; Laboratório de Transformação de Plantas e Análise Em Microscopia (LTPAM), Departamento de Biologia Celular e Genética, Universidade Federal Do Rio Grande Do Norte (UFRN), Natal, RN, Brazil; Programa de Pós-graduação Em Bioquímica e Biologia Molecular, Centro de Biociências, UFRN, Natal, RN, Brazil. Electronic address: katia.scortecci@ufrn.br.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2025 Jan 30; Vol. 337 (Pt 3), pp. 118932. Date of Electronic Publication: 2024 Oct 10.
DOI: 10.1016/j.jep.2024.118932
Abstrakt: Ethnopharmacological Relevance: Talisia esculenta is a fruit tree commonly found in various regions of Brazil. Its fruit is consumed by the local population, and the leaves are used in infusions within traditional Brazilian medicine. These infusions are employed to alleviate pathological conditions such as rheumatic diseases and hypertension, both of which are strongly linked to oxidative stress and chronic inflammation. The investigation of plant extracts represents a promising field of research, as bioactive compounds abundant in plants exhibit pharmacological effects against a variety of pathological conditions.
Aim of the Study: To investigate the antioxidant, anti-inflammatory activities, and toxicity of the infusion and hydroethanolic extracts of T. esculenta leaves (IF and HF) and fruit peels (IC and HC).
Materials and Methods: Initially, the cytotoxicity and the effects of the extracts on oxidative stress in RAW264.7 macrophages were assessed through exposure to H₂O₂, as well as their impact on NO production in RAW264.7 macrophages exposed to LPS. Additionally, the toxicity and ROS production in zebrafish larvae were evaluated using two oxidative stress inducers: H₂O₂ and CuSO₄ combined with ascorbate.
Results: The MTT assay indicated that the extracts exhibited low cytotoxicity, with HF and IF demonstrating protective effects against H₂O₂ exposure. HC reduced NO production in macrophages by 30%. The zebrafish analysis showed that all four T. esculenta extracts (100 μg/mL) were non-toxic, as they did not affect the survival, heart rate, or body size of the animals. Furthermore, all extracts were capable of reducing ROS levels in zebrafish larvae exposed to the H₂O₂ stressor. Notably, ROS reduction by HF, IF, and HC extracts exceeded 50% compared to the positive control (H₂O₂ alone). T. esculenta extracts also demonstrated a significant ability to reduce ROS levels in zebrafish larvae exposed to CuSO₄, with a 70% reduction observed for leaf extracts and over 30% for fruit peel extracts.
Conclusion: This study demonstrated that T. esculenta extracts exhibit significant activity against oxidative damage and contain components with anti-inflammatory properties. Among the extracts, those obtained from leaves were the most effective in providing oxidative protection, supporting the traditional use of leaf infusions.
Competing Interests: Declaration of competing interest X The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Katia Castanho Scortecci reports financial support was provided by Federal University of Rio Grande do Norte. Katia castanho scortecci reports financial support was provided by National Council for Scientific and Technological Development. Maria Lucia da Silva Cordeiro reports financial support was provided by Coordination of Higher Education Personnel Improvement. Ariana Pereira da Silva reports financial support was provided by Coordination of Higher Education Personnel Improvement. Maylla Maria Correia Leite Silva reports financial support was provided by Coordination of Higher Education Personnel Improvement. Hugo Alexandre Oliveira Rocha reports financial support was provided by National Council for Scientific and Technological Development. Weslley de Souza Paiva reports financial support was provided by National Council for Scientific and Technological Development. Ana Carolina Luchiari reports financial support was provided by National Council for Scientific and Technological Development. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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