A TaqMan real-time PCR assay for detection of qacEΔ1 gene in Gram-negative bacteria.

Autor: Kovalchuk SN; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia. Electronic address: s.n.kovalchuk@gmail.com., Arkhipova AL; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia., Bondar SV; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia., Konanov DN; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia., Krivonos DV; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia., Chulkova PS; Pediatric Research and Clinical Center for Infectious Diseases, 197022 Saint Petersburg, Russia., Ageevets VA; Pediatric Research and Clinical Center for Infectious Diseases, 197022 Saint Petersburg, Russia., Fedorova LS; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia., Ilina EN; Research Institute for Systems Biology and Medicine, 117246 Moscow, Russia.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2024 Oct 11; Vol. 227, pp. 107054. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1016/j.mimet.2024.107054
Abstrakt: The transfer of biocide and antibiotic resistance genes by mobile genetic elements is the most common mechanism for rapidly acquiring and spreading resistance among bacteria. The qacEΔ1 gene confers the resistance to quaternary ammonium compounds (QACs). It has also been considered a genetic marker for the presence of class 1 integrons associated with multidrug-resistant (MDR) phenotypes in Gram-negative bacteria. In this study, a TaqMan real-time PCR assay was developed to detect the qacEΔ1 gene in Gram-negative bacteria. The assay has a detection limit of 80 copies of the qacEΔ1 gene per reaction. No false-positive or false-negative results have been observed. Simultaneous amplification and detection of the 16S rRNA gene is performed as an endogenous internal amplification control (IAC). The TaqMan real-time PCR assay developed is a rapid, sensitive, and specific method that could be used to monitor resistance to QACs, the spread of class 1 integrons, and the prediction of associated MDR phenotypes in Gram-negative bacteria.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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