Examination of the anabolic activity and mechanisms of action of the combination of Diosgenin and Ecdysterone in C2C12 myotubes.

Autor: Kostov T; German Sport University Cologne, Department of Molecular and Cellular Sport Medicine, Cologne, Germany., Diel P; German Sport University Cologne, Department of Molecular and Cellular Sport Medicine, Cologne, Germany., Isenmann E; German Sport University Cologne, Department of Molecular and Cellular Sport Medicine, Cologne, Germany; IST University of Applied Sciences, Department of Fitness and Health, Dusseldorf, Germany. Electronic address: e.isenmann@dshs-koeln.de.
Jazyk: angličtina
Zdroj: Toxicology letters [Toxicol Lett] 2024 Nov; Vol. 401, pp. 181-189. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1016/j.toxlet.2024.10.005
Abstrakt: Plant steroids such as ecdysterone (ECDY) or diosgenin (DIO) have been associated with anabolic and performance-enhancing effects for years. However, the molecular mechanisms have not yet been extensively studied in skeletal muscle cells. Consequently, the anabolic activity and associated molecular mechanisms of ECDY and DIO alone and in combination were investigated in C2C12 myotubes. Dose-dependent effects of both compounds on myotube diameter, mRNA expression of IGF-1 and PI3KR1 as well as expression of myosin heavy chain (MHC) proteins were analyzed in differentiated C2C12 cells. In addition, the binding affinities to androgen and estrogen receptors were analyzed. Treatment with ECDY and DIO significantly induced hypertrophy of C2C12 myotubes. Partially additive effects were observed. This is supported by the mRNA expression of IGF-1 and PI3KR1 as well as in the expression of MHC. However, no clear statement can be made regarding which combination has the strongest additive effects. Besides the results suggest that, in contrast to ECDY, DIO has antiandrogenic effects and bind on AR. Consequently, it indicate that two different mechanisms of action are activated in ECDY and DIO combinations. However, this must be confirmed in further cell cultures studies and human interventions concerning anti-doping regulations.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Eduard Isenmann reports financial support was provided by World Anti-Doping Agency. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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