Structural characteristics of a purified Evodiae fructus polysaccharide and its gastroprotection and relevant mechanism against alcohol-induced gastric lesions in rats.

Autor: Wang XY; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: xywang@gmu.edu.cn., Hao M; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: hm48922200@gmu.edu.cn., Li YP; Scientific Research Center, Gannan Medical University, Ganzhou 341000, China. Electronic address: liyanping1@gmu.cn., Zhang J; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: zhangjun7@gmu.cn., Xu QS; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: xuquansheng1@gmu.cn., Yang F; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: yangfan37@gmu.cn., Yang ZC; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: yangzichao1@gmu.cn., Xiong YR; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: xiongyurou2@gmu.cn., Gong ES; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: esgong0201@gmu.edu.cn., Luo JH; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: luojh1212@gmu.edu.cn., Zou Q; School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. Electronic address: Bruce2985@gmu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 281 (Pt 3), pp. 136410. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1016/j.ijbiomac.2024.136410
Abstrakt: Evodiae fructus polysaccharide (EFP) has been previously shown to protect against alcohol-induced gastric lesions. However, which and how active fractions in EFP exert gastroprotection remains unclear. This study aimed to characterize the structure of the purified fraction (EFP-2-1) of EFP, and investigate its gastroprotection and underlying mechanisms. EFP-2-1 was obtained through column chromatography, and was characterized using instrumental analytical techniques. Gastroprotective effect of EFP-2-1 was evaluated using alcohol-induced gastric lesions in rats, and its mechanism was explored through proteomics, metabolomics and diversity sequencing. Results showed that EFP-2-1 had a molecular weight of 7.3 kDa, and consisted mainly of rhamnose, galacturonic acid, galactose and arabinose. Its backbone contained HG and RG-I domains, and branched with →5)-α-l-Araf-(1→, α-l-Araf-(1→ and →4)-β-d-Galp-(1→ residues. EFP-2-1 reduced gastric lesions and the levels of MDA, TNF-α and IL-6, activated PPARγ, primarily altered protein digestion and absorption and bile secretion metabolic pathways, regulated gut microbiota like Faecalibaculum and Lachnoclostridium, and increased short-chain fatty acids production. Correlations were observed among the gut microbiota, metabolites and biochemical indexes influenced by EFP-2-1. These findings suggest that EFP-2-1 is an active fraction of EFP for protecting against alcohol-induced gastric lesions, which may be linked to PPARγ activation, gut microbiota and serum metabolism.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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