ATR-FTIR spectroscopy to evaluate serum protein expression in a murine cerebral ischemia model.

Autor: Montes-Narváez O; Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, Plaza Hidalgo S/N. San Nicolas Panotla, C.P. 90140 Tlaxcala, Mexico; Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala 90000, Mexico., García-Juárez M; Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, Plaza Hidalgo S/N. San Nicolas Panotla, C.P. 90140 Tlaxcala, Mexico. Electronic address: mgarciaj@uatx.mx., Beltrán-Pérez G; Facultad de Ciencias Físico-Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico., Espinosa-García C; Department of Neurology, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Neurology, Yale University School of Medicine, New Haven, CT 06510, USA., González-Flores O; Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala-CINVESTAV, Plaza Hidalgo S/N. San Nicolas Panotla, C.P. 90140 Tlaxcala, Mexico., Delgado-Macuil RJ; Instituto Politécnico Nacional, Centro de investigación en Biotecnología Aplicada, Tepetitla, Tlaxcala 90700, Mexico.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Oct 09; Vol. 326, pp. 125261. Date of Electronic Publication: 2024 Oct 09.
DOI: 10.1016/j.saa.2024.125261
Abstrakt: Stroke is a prevalent vascular disease that causes disability and death worldwide. Molecular techniques have been developed to assess serum concentrations of biomarkers associated with this disease, such as some proteins. ATR-FTIR was proposed as an alternative technique to determine protein expression during the early stages of stroke. Serum samples from sham, ischemic, and ischemic treated with estradiol benzoate (EB; as a neuroprotective agent) male rats were evaluated at 0, 2-, 4-, 6-, 12-, and 24-hours post-ischemia. The analysis was developed in the mid-infrared region but mainly focused on the protein region (1500-1700 cm -1 ), where it was possible to observe the modulation in the absorbance intensity. The peaks at 1545, 1645, 1635, and 1650 cm -1 associated with amide II, amide I, β-sheets, and α-helixes, respectively, were prominent peaks where protein modulation was observed. The results demonstrate that infrared spectroscopy could be a good alternative technique to determine the modulation of protein expression during stroke events.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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