Methods for Imaging Intracellular Calcium Signals in the Mouse Mammary Epithelium in Two and Three Dimensions.
| Autor: | Folacci M; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Chalmers SB; Department of Biomedicine, Aarhus University, Aarhus, Denmark., Davis FM; Department of Biomedicine, Aarhus University, Aarhus, Denmark. felicity@biomed.au.dk.; School of Biomedical Sciences, University of New South Wales, Sydney, NSW, Australia. felicity@biomed.au.dk.; Aarhus Institute of Advanced Studies, Aarhus University, Aarhus, Denmark. felicity@biomed.au.dk.; Danish Research Institute of Translational Neuroscience, Aarhus University, Aarhus, Denmark. felicity@biomed.au.dk.; School of Pharmacy, University of Queensland, Brisbane, QLD, Australia. felicity@biomed.au.dk. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2861, pp. 195-212. |
| DOI: | 10.1007/978-1-0716-4164-4_15 |
| Abstrakt: | The mammary gland has a central role in optimal mammalian development and survival. Contractions of smooth muscle-like basal (or myoepithelial) cells in the functionally mature mammary gland in response to oxytocin are essential for milk ejection and are tightly regulated by intracellular calcium (Ca 2+ ). Using mice expressing a genetically encoded Ca 2+ indicator (GCaMP6f), we present in this chapter a method to visualize at high spatiotemporal resolution changes in intracellular Ca 2+ in mammary epithelial cells, both in vitro (2D) and ex vivo (3D). The procedure to optimally prepare mammary tissue and primary cells is presented in detail. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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