Absence of genotoxicity following pulmonary exposure to metal oxides of copper, tin, aluminum, zinc, and titanium in mice.

Autor: Gutierrez CT; National Research Centre for the Working Environment, Copenhagen Ø, Denmark.; Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark., Hadrup N; National Research Centre for the Working Environment, Copenhagen Ø, Denmark.; Research group for risk-benefit, National Food Institute, Technical University of Denmark., Loizides C; Climate and Atmosphere Research Centre, The Cyprus Institute, Nicosia, Cyprus., Hafez I; Climate and Atmosphere Research Centre, The Cyprus Institute, Nicosia, Cyprus., Biskos G; Climate and Atmosphere Research Centre, The Cyprus Institute, Nicosia, Cyprus.; Faculty of Civil Engineering and Geosciences, Delft University of Technology, Delft, The Netherlands., Roursgaard M; Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark., Saber AT; National Research Centre for the Working Environment, Copenhagen Ø, Denmark., Møller P; Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark., Vogel U; National Research Centre for the Working Environment, Copenhagen Ø, Denmark.; National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark.
Jazyk: angličtina
Zdroj: Environmental and molecular mutagenesis [Environ Mol Mutagen] 2024 Oct; Vol. 65 (8), pp. 251-260. Date of Electronic Publication: 2024 Oct 12.
DOI: 10.1002/em.22634
Abstrakt: Inhalation of nanosized metal oxides may occur at the workplace. Thus, information on potential hazardous effects is needed for risk assessment. We report an investigation of the genotoxic potential of different metal oxide nanomaterials. Acellular and intracellular reactive oxygen species (ROS) production were determined for all the studied nanomaterials. Moreover, mice were exposed by intratracheal instillation to copper oxide (CuO) at 2, 6, and 12 μg/mouse, tin oxide (SnO 2 ) at 54 and 162 μg/mouse, aluminum oxide (Al 2 O 3 ) at 18 and 54 μg/mouse, zinc oxide (ZnO) at 0.7 and 2 μg/mouse, titanium dioxide (TiO 2 ) and the benchmark carbon black at 162 μg/mouse. The doses were selected based on pilot studies. Post-exposure time points were 1 or 28 days. Genotoxicity, assessed as DNA strand breaks by the comet assay, was measured in lung and liver tissue. The acellular and intracellular ROS measurements were fairly consistent. The CuO and the carbon black bench mark particle were potent ROS generators in both assays, followed by TiO 2 . Al 2 O 3 , ZnO, and SnO 2 generated low levels of ROS. We detected no increased genotoxicity in this study using occupationally relevant dose levels of metal oxide nanomaterials after pulmonary exposure in mice, except for a slight increase in DNA damage in liver tissue at the highest dose of CuO. The present data add to the body of evidence for risk assessment of these metal oxides.
(© 2024 The Author(s). Environmental and Molecular Mutagenesis published by Wiley Periodicals LLC on behalf of Environmental Mutagenesis and Genomics Society.)
Databáze: MEDLINE
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