Amplification-free detection of Ascochyta blight in chickpea using a simple molecular beacon assay.

Autor: Dyussembayev K; School of Environment and Science, Griffith University, Nathan, QLD, 4111, Australia.; L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan., Akpe V; School of Environment and Science, Griffith University, Nathan, QLD, 4111, Australia., Yechshzhanov T; Pedagogical Institute, Astana International University, Astana, 010000, Kazakhstan., Cheesman MJ; Menzies Institute, Griffith University, Nathan, QLD, 4111, Australia.; School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, QLD, 4222, Australia., Kim TH; School of Environment and Science, Griffith University, Nathan, QLD, 4111, Australia.; Queensland Micro and Nanotechnology Centre, Griffith University, Nathan, QLD, 4111, Australia., Cock IE; School of Environment and Science, Griffith University, Nathan, QLD, 4111, Australia. I.Cock@griffith.edu.au.; Centre for Planetary Health and Food Security, Griffith University, Nathan, QLD, 4111, Australia. I.Cock@griffith.edu.au.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 11; Vol. 14 (1), pp. 23846. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1038/s41598-024-74564-5
Abstrakt: Ascochyta blight is a major biotic stress that limits chickpea production globally. Fungicide application remains one of the effective control measures for the endemic spread. Due to the serious threat that synthetic fungicides pose to crop quality, early diagnosis of the pathogen is imperative. Whilst there have previously been several conventional lab-based diagnostic methods developed for early detection of Ascochyta rabiei, they require long assay times, specialised equipment and facilities, and trained personnel to process the samples. To overcome this challenge, a rapid amplification-free detection assay using a molecular beacon probe was developed. The method consists of a simple assembly assay that accurately detects pathogen within 30 min. The developed assay is species-specific and has a similar sensitivity level as conventional amplification-based methods. Although it is still a lab-based technique, considering the simplicity of the assay, it has a great potential to be developed further as a reliable in-field diagnostic device for early detection and quantification of fungal pathogen spores.
(© 2024. The Author(s).)
Databáze: MEDLINE
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