Diversity and Bioactivity of Endophytic Actinobacteria Associated with the Roots of Artemisia herba-alba Asso from Algeria.

Autor: Djemouai N; Département de Biologie, Faculté des Sciences de la Nature et de la Vie et Sciences de la Terre, Université de Ghardaia, BP 455, 47000, Ghardaïa, Algeria. djemouai.nadjette@univ-ghardaia.dz.; Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure Cheikh Mohamed El Bachir El Ibrahimi, B.P. 92, 16050, Kouba, Algiers, Algeria. djemouai.nadjette@univ-ghardaia.dz., Meklat A; Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure Cheikh Mohamed El Bachir El Ibrahimi, B.P. 92, 16050, Kouba, Algiers, Algeria., Youcef KOH; Unité de Recherche Appliquée en Energies Renouvelables (URAER), Centre de Développement des Energies Renouvelables (CDER), Ghardaïa, Algeria., Nacer A; Equipe Biologie des Sols, Laboratoire de Biologie et Physiologie des Organismes, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumediene (USTHB), El-Alia, Bab Ezzouar, BP32, 16111, Algiers, Algeria.; Laboratory of Molecular Biology, Cluster of Plant Developmental Biology, Plant Science Group, Wageningen University and Research (WUR), Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands., Saadi SA; Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure Cheikh Mohamed El Bachir El Ibrahimi, B.P. 92, 16050, Kouba, Algiers, Algeria., Verheecke-Vaessen C; Magan Centre of Applied Mycology, Cranfield University, Cranfield, MK43 0AL, UK.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2024 Oct 11; Vol. 81 (12), pp. 402. Date of Electronic Publication: 2024 Oct 11.
DOI: 10.1007/s00284-024-03932-1
Abstrakt: The isolation of endophytic actinobacteria from the roots of wild populations of Artemisia herba-alba Asso, a medicinal plant collected from the arid lands of Algeria, is reported for the first time. Forty-five actinobacterial isolates were identified by molecular analysis and in vitro evaluated for antimicrobial activity and plant growth-promoting (PGP) abilities (1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, nitrogen fixation, phosphate and potassium solubilization, ammonia, and siderophores production). The phylogenetic relationships based on 16S rRNA gene sequences show that the genus Nocardioides (n = 23) was dominant in the sampled localities. The remaining actinobacterial isolates were identified as Promicromonospora (n = 11), Streptomyces (n = 6), Micromonopora (n = 3), and Saccharothrix (n = 2). Only six (13.33%) strains (five Streptomyces and one Saccharothrix species) were antagonistic in vitro against at least one or more indicator microorganisms. The antimicrobial activity of actinobacterial strains targeted mainly Gram-positive bacteria. The results demonstrate that more than 73% of the isolated strains had ACC deaminase activity, could fix atmospheric nitrogen and were producers of ammonia and siderophores. However, only one (2.22%) strain named Saccharothrix sp. BT79 could solubilize phosphorus and potassium. Overall, many strains exhibited a broad spectrum of PGP abilities. Thus, A. herba-alba provides a source of endophytic actinobacteria that should be explored for their potential biological activities.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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